中文摘要：

以渤西油田海上平台中质原油作为研究对象,进行岸滩模拟溢油生物降解实验.采用气相色谱与质谱联用技术（GC-MS）对原油中双环倍半萜化合物进行了分析,探讨了不同生物降解条件下双环倍半萜化合物的分布规律,并对该化合物诊断比值的变化规律以及稳定性进行了考察.研究结果表明,经过21 d的生物降解,加入生物降解菌剂和营养盐的原油中双环倍半萜化合物的分布变化不大,加入生物降解菌剂和缓释肥的原油中双环倍半萜化合物降解比较大.经过t检验分析,经过21 d短期生物降解,双环倍半萜化合物诊断比值DR3、DR6、DR9以及DR10,可以用于判别生物降解前21 d内油样是否来自同一油源.双环倍半萜化合物诊断比值对于溢油来源鉴别具有重要意义.

英文摘要：

Using the middle crude oil from the west offshore platform of the Bohai sea, coastal oil spill biodegradation was simulated. Based on the GC-MS analytical results, the distribution pattern of bicyclic sesquiterpene during short-term biodegradation was investigated, and the change and stability of the diagnosis ratios of these compounds were evaluated. The results show that the patterns of bicyclic sesquiterpene in the crude oils changed greatly after 21 d biodegradation with the use of oil-degrading agent and slow release fertilizer （SRF）. By comparison, the distribution of bicyclic sesquiterpene without the addition of oil-degrading agent and SRF changed little. After t-test analysis, the diagnosis ratios DR3, DR6, DR9 and DR10 had no apparent change, and therefore could be used as an indicator to decide whether the source of the oil samples during the first 21 days biodegradation are the same. The results suggest that the bicyclic sesquiterpenes can be used as a diagnostic tool for oil spill source identification.