以酪胺为模板分子,丙烯酰胺(AM)为功能单体,乙二醇二甲基丙烯酸酯(EDGMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,在制孔剂甲醇中,采用本体聚合法制备酪胺分子印迹聚合物。对预组装体系的紫外光谱分析表明:1个酪胺主要与1个AM分子形成TYR—AM型复合物。通过静态平衡吸附试验和选择性试验研究印迹聚合物的吸附性能和选择性,并采用Scatchard模型研究印迹聚合物的结合特性。结果表明,印迹聚合物的最大表观吸附量Qmax=248.33μmol/g,平衡解离常数KD=1.76μmol/mL。
The tyramine molecularly imprinted polymers were prepared in methanol solvent with tyramine as the template, acrylamide (AM) as functional monomer, ethylene glycol dimethaerylate (EDG-MA) as cross-linker, azobisisobutyronitrile (AIBN) as initiator. The UV spectrophotometry was used to analyze the interaction between tyramine and AM. The results showed that one tyraraine was entrapped by one AM. Static equilibrium adsorption test and selective test were used to study the adsorption, selectivity and binding prop erties, moreover, Seachard model was used to study binding proper- ties of these molecularly imprinted polymers. The results showed that the maximum apparent adsorption capacity and the equilibrium dissociation constant (KD)of this molecularly imprinted polymers (Qmax) were 248.33 μmol/g and 1.76 μmol/mL respectively.