采用盆栽试验,研究了不同氮肥用量[0、200、300和400 mg/kg CO(NH2)2]对Cd胁迫(50 mg/kg)下结缕草光合及叶绿素荧光特性的影响。结果表明:不同浓度的氮肥均能使Cd胁迫下结缕草叶片叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、胞间二氧化碳浓度(Ci)、蒸腾速率(Tr)、光化学猝灭系数(q P)、叶片PSⅡ最大光量子产量(Fv/Fm)及表观光电子传递效率(ETR)有所提高,而非光化学猝灭系数(NPQ)有所降低,说明氮肥对Cd污染具有一定的缓释作用。从结缕草叶片叶绿素(叶绿素a、叶绿素b、叶绿素a+b)含量、气孔导度、胞间二氧化碳浓度、叶片PSⅡ最大光量子产量及表观光电子传递效率指标来看,以氮肥浓度300 mg/kg缓解作用最大,200 mg/kg次之,400 mg/kg最弱;相对于叶片净光合速率、蒸腾速率、水分利用率(WUE)、非光化学猝灭系数来说,以氮肥浓度300 mg/kg缓解作用最为明显,400 mg/kg次之,200 mg/kg最弱;不同施肥浓度及镉胁迫对结缕草叶片光化学猝灭系数基本无影响(P〉0.05)。通过本研究得出:施用氮肥对Cd胁迫下结缕草具有一定的缓解作用,当氮肥浓度为300 mg/kg时缓解效果最为显著。
The method of pot culture was used to investigate the proper application rate of the nitrogen fertilizer during the period of under cadmium stress (50 mg/kg) plant growth. The study was carried out to examine the effects of different dosages nitrogen fertilizer [ 0, 200, 300 and 400 mg/kg CO ( NH2 ) 2 ] on photosynthesis and fluorescence characteristics of Zoysia japonica under cadmium stress. The results showed that different dosages nitrogen fertilizer enhanced chlorophyll contents, net photosynthetic rate, stomata] conduction, intercellular CO2 concentration, transpiration rate, photochemical quenching, maximum photo quantum yield, and the electron transfer rate and decreased non-photochemical quenching (NPQ) under Cd stress, which demonstated that N fertilizer could release the effect of Cd stress on Zoysia japonica. For the effects of different nitrogen concentration on chlorophyll content of Zoysia (Chl a, Chl b, Chl a + b), stomata] conductance (Gs), intercellular carbon dioxide concentration ( Ci ), ]ear maximum quantum yield of PS Ⅱ ( Fv / Fro) and the electron transfer rate ( ETR), it showed that treatment with 300 mg/kg nitrogen was the best, then 200 and 400 mg/kg. For the effects of different nitrogen concentration on net photosynthetic rate (Pn), transpiration rate (Tr), water use efficiency (WUE) and non-photochemical quenching (NPQ), the result was that treatment with 300 mg/kg nitrogen was better than that of 400 and 200 ing/kg. However photochemical quenching (qP) was not significantly affected (P 〉0. 05) by nitrogen concentration. It was concluded that 300 mg/kg nitrogen concentration had the greatest effect on Zoysia japonica under cadmium stress in this study.