中文摘要：

目的：探讨益气化瘀方对椎间盘软骨细胞凋亡状态下Bax、Bcl-2及Caspase-8表达的调控作用。方法：清洁级雄性1月龄SD大鼠20只，酶消化法获取椎间盘软骨细胞，用抗Fas抗体诱导其发生凋亡，通过益气化瘀方及其拆方益气方、化瘀方药理血清干预，免疫组化法（SP）检测分析大鼠椎间盘软骨细胞不同状态下Bax、Bcl-2及Caspase-8的表达。结果：相对正常组，凋亡模型组的Bax和Caspase-8表达明显，而Bcl-2轻度表达，有显著差异（P〈0．01）。益气化瘀方、益气方和化瘀方均能降低椎间盘软骨细胞的Bax、Caspase-8的表达，而上调Bcl-2的表达，3种中药组与诱导凋亡组相比有显著差异（P〈0．01）。结论：中药益气化瘀方、益气方和化瘀方都可以调控凋亡的大鼠椎间盘软骨细胞内Bax、Bcl-2、Caspase-8的表达，其治疗颈椎病的机制与其对凋亡相关因子的调控有关。

英文摘要：

Objective： To study the effects of Yiqi-Huayu recipe （益气化瘀方） and its decomposed formulas-medicated on expressions of Bcl-2, Bax and Caspase-8 of apoptotic discus intervertebrale chondrocytes in rats. Methods： Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi-Huayu recipe, Yiqi recipe, Huayu recipe-medicated sera on the expressions of Bcl-2, Bax and Caspase-8 of apoptotic discus intervertebrale chondrocytes in rats induced by anti-Fas antibody. Results： As compared with apoptosis group, Bcl-2 expression was higher, Bax and Caspase-8 expressions were lower in Yiqi-Huayu-treated, Yiqi-treated, Huayu-treated （ P 〈 0.01 ）. Conclusion： Yiqi-Huayu recipe and its decomposed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of Bcl-2, Bax and Caspase-8.