在在 vitro 估计的兔子 osteoctastsis 的骨头再吞活动的 icariin 的效果。骨破折从日本白兔子被孤立并且在有消毒骨头片在的盘子上有教养各好。在有在各种各样的集中的 icariin 的处理以后,骨破折的骨头再吞活动被检验坑区域,肌动朊的产生,尺寸和数字包围的超级氧化物阴离子(~· O _2~-) 和细胞内部的钙集中评估[Ca~(2+)]_i。是由这些数据揭示了, icariin 得到了连续衰落[Ca~(2+)]_i,压缩的做的肌动朊戒指和 ~· O _2~- 产生减少了。这些事件导致了更小并且由 icariin 显示兔子骨破折的压制的骨头再吞活动的更少坑。
The effect of icariin on the bone resorption activity of rabbit osteociasts is assessed in vitro. Osteoclasts were isolated from Japanese white rabbits and cultured on plates with a sterilized bone slice in each well. After treatment with icariin at various concentrations, the bone resorption activity of osteoclasts was evaluated by examining pit areas, superoxide anion (^·O2^-) generation, size and number of actin rings and intracellular calcium concentration [Ca^2+]i. As revealed by these data, icariin elicited continuous decline of [Ca^2+]i, making actin ring constricted and ^·O2^- generation decreased. These events resulted in smaller and fewer pits which indicate suppressed bone resorption activity of rabbit osteoclasts by icariin.