中文摘要：

目的探讨重组腺相关病毒（rAAV）介导大鼠血红素氧合酶-1（rHO—1）基因转染对心肌缺血／再灌注（I／R）损伤大鼠心肌细胞凋亡的影响。方法95只体重225～250g的雄性SD大鼠随机分为假手术组（SO组，n-8）、生理盐水组（NS组，n=29）、rAAV-荧光蛋白组（rAAV—EGFP组，n=29）及rAAV—rHO-1组n=29）。NS组、rAAV—EGFP组和rAAV—rHO-1组分别于大鼠左心室前后壁共取4点分别注入生理盐水、rAAv—EGFP或rAAV—rHO-1病毒液共600μl。在基因转染后3个月，各组处死3只大鼠，取注射部位心肌，荧光显微镜下观察荧光蛋白的表达并计算转染率；用免疫组化染色和逆转录-聚合酶链反应（RT—PCR）检测HO-1的蛋白和mRNA表达。采用结扎左冠状动脉前降支30min、再灌注120min建立心肌I／R模型；成模后处死大鼠测定其心肌梗死面积及心肌细胞凋亡指数（AI），光镜下观察心肌组织病理学改变。结果荧光显微镜下仅rAAV—EGFP组可见心肌有荧光蛋白表达，转染率为（53．54±2．0）％。与SO组比较，NS组、rAAV—EGFP组和rAAV—rHO-1组AI增加（P均〈0．01）；与NS组和rAAV—EGFP组比较，rAAV—rHO-1组HO—1的蛋白及mRNA表达增加，心肌梗死面积减小，AI减少（P均〈0．01）；NS组及rAAV—EGFP组心肌组织病理学损伤较SO组和rAAV—rHO-1组为重。NS组与rAAV—EGFP组间上述指标比较无统计学意义。结论rAAV介导的rHO-1基因转染大鼠心肌细胞后，能够显著减少心肌细胞凋亡，从而减轻心肌I／R损伤。

英文摘要：

Objective To investigate the effect of rat hemeoxygenase-1 （rHO-1） gene carried by recombinant adeno-associated virus （rAAV） on myocardial ischemia/reperfusion （I/R） injury in rats. Methods Ninety-five healthy male Sprague-Dawley （SD） rats weighing 225-250 g were randomly divided into four groups： sham operation group （ I , n=8）; normal saline group （ Ⅰ , n=29）; rAAV-EGFP （enhan ced green fluorescent protein） group （Ⅲ , n=29） and rAAV-rHO-1 group （Ⅳ, n=29）. In Ⅰ , Ⅱ and Ⅳ groups, 600μl of normal saline, rAAV-EGFP or rAAV-rHO-1 was injected intramyocardially at four sites on the anterior and posterior walls of left ventricle. After 3 months, 3 animals in each group were sacrificed. EGFP-expression in heart sections was observed under fluorescence microscope. The expression of HO-1 in the injected myocardium was detected by immunohistochemistry and reverse transeription-polymerase chain reaction （RT-PCR）. The remaining animals in the four groups were anesthetized, tracheostomized and mechanically ventilated. I/R of myocardium was producing by blocking the left anterior descending branch of coronary artery （LAD） for 30 minutes followed by 120 minutes reperfusion. After the successful reproduction of the model, the animals were killed and their hearts were harvested for determination of myocardial infarct size, apoptotic index （AI）, and pathology changes in myocardial tissue. Results The expression of EGFP was detected in group Ⅲ only, and transfeetion efficiency was （53.5±2.0）%. AI was significantly higher in group Ⅰ , group Ⅲ and group Ⅳ than in group Ⅰ （all P〈0.01）. The expression of HO-1 mRNA and protein was significantly higher, and the infarct size and AI were significantly lower in group Ⅳ than in group Ⅱ and group Ⅲ （all P〈0.01）. The degree of damage to myocardial tissue was significantly severer in group Ⅱ and group Ⅲ than in group Ⅰ and group Ⅳ. There was no significant difference between group Ⅱ and gr