中文摘要：

为制备免疫原性较好的苊烯完全抗原，以3-苊烯丁酸（ACE）为原料，分别采取活化酯法和混合酸酐法与牛血清白蛋白（BSA）和鸡卵清白蛋白（OVA）偶联，制备免疫原和包被原，经非变性聚丙烯酰胺凝胶电泳和紫外光谱吸收法鉴定偶联结果。足垫免疫BALB／C小鼠，间接ELISA检测血清效价。结果表明：BSA（OVA）、过程载体、BSA（OVA）-ACE在Native—PAGE中迁移率发生明显改变，紫外光谱扫描发现3种物质在最大吸收峰处波长发生明显偏移，表明2种方法制备的苊烯完全抗原偶联成功。间接ELISA检测血清效价最高为1：256000，可以进行苊烯单克隆抗体的制备。

英文摘要：

In order to prepare a complete antigen of acenaphthylene with better immunogenicity, the complete antigen was synthesized by ACE-3-butyricaid and bovine serum albumin （BSA） or chicken ovalbumin （OVA） using active ester method and mixed anhydride method, respectively as immunogen and coating antigen. Coupled productions were identified by native polyacrylamide gel electrophoresis （PAGE） and ultraviolet spectrum method. BALB/C mice were immunized by the immunogen. The titer of anti-acenaphthylene polyclonal antibody was detected by indirect ELISA. The results showed that： The migration rates of BSA（ OVA）, Process carrier and BSA（ OVA） -ACE in N- ative-PAGE obviously changed. The wavelengths of them in the maximum absorption peak had obvi- ous deviation. Immunogen and coating antigen of ACE by active ester method and mixed anhydride method were successfully synthesized. The highest serum titer was 1 ： 256 000, and the anti-acenaphthylenemonoclonal antibody could be prepared.