中文摘要：

目的 探讨核心结合因子a1、骨形成蛋白和骨桥素3者在小鼠牙周组织发育过程中的时间和空间分布特点、表达意义及它们之间的相互作用。方法 用SABC免疫组化法检测3者在出生后不同时期小鼠牙周组织发育中的表达及特点。结果牙齿发育早期，仅骨形成蛋白在牙囊细胞中表达：当牙根开始发育后，3者皆在牙周膜细胞、成牙骨质细胞中表达，但骨形成蛋白在无细胞牙骨质和细胞牙骨质中皆不表达，核心结合因子a1在细胞牙骨质中表达，而骨桥素则在无细胞牙骨质、细胞牙骨质和牙槽骨表面皆呈强阳性表达。结论 核心结合因子a1、骨形成蛋白、骨桥素3者皆参与牙周组织的发育，对矿化和生长发育发挥重要作用。

英文摘要：

Objective To study the expression and interaction of core binding factor al （Cbfal）, bone morphogenetic proteins （BMPs） and osteopontin （OPN） in the developing periodontal tissues of mice. Methods A mice developing periodontal tissues study model was created histologically by 5-27 day postnatal BALB/c mice, then the immunohistochemical localization of Cbfal, BMPs and OPN in different developing stages were undertaken. Results In early stage of postnatal mice periodontal tissues development, only BMPs expressed in dental follicle cells, though the signal was weak. When root was forming, all of them were expressed in periodontal ligament cells and cementoblasts, while only OPN in acellular cementum, cellular cementum and the surface of alveolar bone, Cbfal only in cellular cementum and BMPs was seen in neither acellular cementum nor cellular cementum. Conclusion Cbfal, BMPs and OPN all involve in the development of periodontal tissues, while OPN is crucial for cementum.