中文摘要：

目的 观察运动疲劳对大鼠海马和纹状体脑源性神经营养因子（BDNF）及胶质纤维酸性蛋白（GFAP）表达的影响，探讨运动疲劳产生的神经生物学机制。方法：选取24只SD大鼠随机分为对照组（CG）和实验组（EG），分别在运动后0，12，24h将大鼠麻醉，灌注制备脑石蜡切片，采用免疫组化染色法观察并分析海马和纹状体BDNF、GFAP表达水平的变化。结果 EG海马锥体细胞排列紊乱疏松，部分细胞出现固缩、肿胀和碎裂；GFAP阳性细胞胞体增大，突起分支增多、增粗，着色加深。海马和纹状体12EG、24EG组BDNF阳性表达显著高于CG（P〈0．05，P〈0．01），且海马24EG显著高于0EG（P〈0．05）。各EG大鼠海马区神经细胞GFAP阳性表达较CG明显增强（P〈0．05，P〈0．01）。结论运 动疲劳引起大鼠海马和纹状体BDNF和GFAP表达水平上调，提示BDNF与GFAP参与了运动疲劳产生的神经生物学调控过程。

英文摘要：

To investigate BDNF and GFAP expression changes in rats＇ hippocampus and striatum after swimming and discuss the neurobiology mechanism of exercise-induced fatigue, 24 rats were divided randomly into CG and EG （0EG, 12EG, 24EG） and BDNF and GFAP expression changes in hippocampus and striatum were observed using SABC method. After exercise-induced fatigue, cells arrange shows foul-up and loosen in cone district of hippocampus. Some cells crinkle, strut and chip. GFAP expression in cell＇ s body becomes augment and shows wider axon. Expression of BDNF in 12EG, 24EG significantly increased compared with CG （P 〈 0.05,P 〈 0.01）, and 24EG also significantly increased compared with 0EG （P 〈 0.05）. Expression of GFAP significantly increased compared with CG （P 〈 0.05,P 〈 0.01）. Exercise- induced fatigues have increased BDNF and GFAP expression. It has been proved that both of BDNF and GFAP may play an important role in the neurobiological modulation for exercise-induced fatigue.