中文摘要：

Orexin 有几生物功能，包括繁殖内分泌的发信号的规定，它收到了许多注意。然而，很少对 orexin 通过调整 neuroendocrine 荷尔蒙和肽的层次的机制被知道。我们注射了 orexin A 或生理进 10 ovariectomized (OVX ) 的侧面的室盐 gilts，并且在浆液 luteinizing 决定了随后的变化由使用放射性免疫测定(RIA ) 的荷尔蒙(LH ) 集中。我们也由使用半量的反向的抄写聚合酶链反应在垂体在视下丘和 LH，刺激滤泡的荷尔蒙(FSH ) ， POMC，和 ghrelin mRNAs 的检验了 GnRH， NPY，和 POMC mRNAs 的表示。我们发现了下列结果：(1 ) Orexin A 短暂地支持了 LH 分泌物；浆液 LH 集中开始在 orexin 注射以后在 10 min 增加，在 30 min 达到顶点，并且回到了它在 1.5 h 的起始的水平；(2 ) 在视下丘的 orexin A upregulated GnRH mRNA 表示和 downregulated NPY 和 POMC mRNAs 表示；(3 ) orexin A upregulated LH 和 FSH mRNAs 表示(FSH， P > 0.05 ) ，但是在垂体的 downregulated ghrelin mRNA 表示。没有重要效果在 FSH 和 POMC mRNAs 的垂体表示上被观察。我们的数据建议 orexin A 由在 NPY， POMC 和 ghrelin 上经由它的效果直接并且间接地刺激 GnRH 和 LH 版本调整繁殖功能表达式。

英文摘要：

Orexin has several biological functions, including the regulation of reproductive endocrine signaling, which has received much attention. However, little is known about the mechanism through which orexin regulates the levels of neuroendocrine hormones and peptides. We injected orexin A or physiological saline into the lateral ventricle of 10 ovariectomized （OVX） gilts, and determined the subsequent changes in serum luteinizing hormone （LH） concentration by using radioimmunoassay （RIA）. We also examined the expression of GnRH, NPY, and POMC mRNAs in the hypothalamus and that of LH, folliclestimulating hormone （FSH）, POMC, and ghrelin mRNAs in the pituitary by using semi-quantitative reverse transcription polymerase chain reaction. We found the following results： （1） Orexin A transiently promoted LH secretion; serum LH concentration started to increase at 10 min after the orexin injection, peaked at 30 min, and returned to its initial level at 1.5 h; （2） orexin A upregulated GnRH mRNA expression and downregulated NPY and POMC mRNAs expression in the hypothalamus; （3） orexin A upregulated LH and FSH mRNAs expression （FSH, P〉 0.05）, but downregulated ghrelin mRNA expression in the pituitary. No significant effects were observed on the pituitary expression of FSH and POMC mRNAs. Our data suggest that orexin A regulates reproductive function by stimulating GnRH and LH release directly and indirectly via its effects on NPY, POMC and ghrelin expression.