中文摘要：

目的观察食用紫菜后的志愿者尿中各形态砷和尿总砷（TAs）的随时间的变化规律及其对尿TAs的影响。方法志愿者禁食海产品至少72h，收集食用紫菜后第1、3、5、8、12、24、36、48、60、72小时尿样；运用横断面调查方法，随机采集某渔村55名居民尿样，所有尿样采用冷阱捕集．氢化物发生原子分光光度法检测尿中各形态砷[一甲基砷（MMA）和二甲基砷（DMA）]和TAs含量。结果志愿者食用紫菜后，尿TAs随时间的延长而上升，在第36小时达到高峰，然后随时间的延长而下降，尿中DMA的变化同尿TAs的变化一致。食用紫菜后36h内女性尿中DMA的含量高于男性（P〈0．05），TAs排泄高峰时间早于男性12h。渔村居民尿中MMA、DMA和尿TAs显著高于对照组居民，渔村居民尿中特异性地检出了三甲基砷（TMA）。结论志愿者食用海产品后，尿TAs会有显著的上升且尿TAs中以DMA的变化为主。女性对海产品中的砷化物的代谢和排泄能力高于男性。渔村居民尿中DMA和TMA为尿TAs的主要组成部分。

英文摘要：

Objective To analyze the variation of urinary arsenic species and urinary total arsenic （TAs） along with time and its impact on urinary TAs after consumption of seafood among volunteers. Methods Forty-seven volunteers （23-26-years old,26 mate,21 female） refrained from eating any seafood for at least 72 h before commencing the seaweed ingestion experiment. The volunteers then consumed seafood in one meal. The urine samples were collected on the 1st, 3rd, 5th, 8th, 12th, 24th, 36th, 48th, 60th and 72nd hour after consumption of seafood. The cross-sectional survey methodology was used, and the urine samples of 55 fishing village residents were randomly selected. All of the urine samples were determined for the urinary arsenic species （MMA and DMA） and TAs by hydride generation atomic absorption spectroscopy. Results After intake of seafood, the levels of TAs in the urine samples of volunteers increased with the duration after intake, reached the peak at 36th hour, then decreased gradually, the same trend was found in DMA. Among female volunteers, higher level of DMA was found in urine samples compared with that in male （P〈0.05）, the peak of TAs appeared 12 h earlier than that of male did. The significantly higher levels of MMA,DMA and TAs were observed in urine samples from fishing village residents, among which the TMA was specially detected ,compared with those from controls. Conclusion After consumption of seafood among volunteers, the levels of TAs in the urine samples, mainly consisting of DMA, increases significantly. Female shows higher capability to perform the metabolism and excretion of arsenide compared with that of male. TAs in the urine samples of fishing village residents mainly consists of DMA and TMA.