中文摘要：

基于光度吸收原理，设计了一种集成片上混合和光纤检测功能于一体的微流控芯片，用于细胞和组织培养过程中乳酸代谢浓度的在线检测。通过光学设计软件（Zemax）优化设计了吸收光路，得到微流控芯片沟道宽度为250μm，利用计算流体动力学软件（CFD）模拟确定了乳酸和显色剂片上混合时微流控芯片沟道的溶液完全混合位置和光纤检测点，采用微电子机械系统（MEMS）加工了基于聚二甲基硅氧烷（PDMS）的微流控芯片，将片上混合和光纤检测功能集成在一个以PDMS和载玻片组成的芯片上。实验结果表明，该芯片成功实现了乳酸和显色剂的片上混合和实时检测，检出限（LOD）为O．52mmol／L（47mg／L），乳酸浓度从1mmol／L变化至5mmol／L时的芯片响应时间为130S，能够满足细胞和组织培养过程中乳酸在线检测要求。

英文摘要：

Based on optical absorption of color solution, a microfluidic chip with functions of mixing on chip and optical fibres detecting was developed to on-line monitoring the lactate during the cell and tissue culture. The channel width that decides the optical transmittance has been optimized to 250 μm through the optical de- sign software （Zemax） , and the full-mixing position and optical fibre detecting position of the channel in microfludic chip used for mixing lactate and its reagent has been decided by simulation with computational fluid dynamics （CFD） software. Therefore, a microfluidic chip comprised by polydimethylsiloxane （PDMS） and slide, with the function of mixing on chip and on-line monitoring by optical fibres, has been achieved through microeletro-mechanical system （MEMS） processing. The experiments show that mixing of lactate and its reagent on chip and the on-line monitoring of lactate can be realized and the LOD of this chip is up to 0.52 mmoL/L（47 mg/L） , the response time is 130 s when the lactate concentration varies from 1 mmoL/L to 5 mmoL/L. This kind of microfluidic chip can meet the need for on-line monitoring of lactate.