中文摘要：

基于8-17 DNA酶的催化切割特性和类辣根过氧化物酶DNA酶的氧化还原反应催化特性,发展了一种新型核酸酶催化放大传感体系,并用于Pb2＋的比色检测.考察了K＋浓度,pH值及反应时间对检测体系的影响.ABTS吸光度变化与Pb2＋浓度在5.0～100 nmol/L范围内呈良好的线性关系,检出限为3.0 nmol/L.此外,因Pb2＋酶的特异性,本方法对Pb2＋具有良好的选择性.

英文摘要：

Based on the catalytic cleavage of 8- 17 DNA enzyme （DNAzyme） and the catalytic redox of horseradish peroxidase （HRP）-mimicking DNAzyme, a novel DNAzyme catalytic amplification biosensing platform has been proposed for the colorimetric detection of lead ions （ Pb2＋ ）. The effects of K＋ concentration, pH value and incubation time on the detection system were investigated. The system exhibited a dynamic response range for Pb2＋ from 5 to 100 nmol/L with a detection limit of 3 nmol/L. In addition, the selectivity of the sensor for Pb2＋ against other environmentally related metal ions was outstanding.