中文摘要：

目的：探讨黄连素（berberine）对巨噬细胞清道夫受体CD36、LOX-1、CD68等表达的影响。方法：以佛波醇酯（PMA）诱导分化的单核源巨噬细胞为细胞模型,黄连素（50μmol/L）预处理1 h后,加入氧化低密度脂蛋白（oxLDL）刺激,采用流式细胞仪检测巨噬细胞表面清道夫受体的表达。结果：单核细胞分化成巨噬细胞后,CD36表达明显升高,而黄连素预处理显著抑制CD36的表达。oxLDL刺激巨噬细胞24 h后,巨噬细胞表面清道夫受体CD36、LOX-1和CD68表达明显升高（P〈0.01）,黄连素预处理后巨噬细胞表面CD36、LOX-1的表达受到抑制,但CD68的表达未受影响（P〉0.05）。结论：黄连素抑制巨噬细胞表面清道夫受体CD36、LOX-1的表达,一定程度上可减少巨噬细胞通过清道夫受体途径对脂质oxLDL的吞噬。

英文摘要：

Objective： To investigate the effect of berberine on the expression of scavenger receptors（CD36,LOX-1,CD68） in macrophages.Methods： PMA-induced macrophages stimulated by oxLDL was used as model in this study to investigate the expression of scavenger receptors,including CD36,CD68 and LOX-1,which was tested using Flow cytometry.Results： Berberine greatly inhibited CD36 expression during monocytes differentiation to macrophages and similar result was obtained in oxLDL-stimulated macrophages.Moreover,in oxLDL-stimu-lated macrophages,LOX-1 expression,but not CD68,was also decreased after berberine treat-ment than those in oxLDL only group.Conclusion： Berberine suppresses the expression of CD36 and LOX-1,but not CD68 in macrophages.