中文摘要：

目的观察表皮细胞在3种培养基中的生长情况，检测不同时间点培养基中内皮素-1（ET-1）和干细胞因子（SCF）的水平。方法采用3种培养基培养表皮细胞，分别在培养24h，48h，72h，96h时收集培养上清，采用酶联免疫吸附试验（ELISA）方法检测ET-1和SCF水平，同时比较不同培养基中细胞的生长状态。结果三组培养上清中，培养基2（M2）中上清ET-1的含量最高；随着时间延长ET-1含量均增加，三组间比较差异有统计学意义（P均〈0．01）；三组培养上清中SCF含量在第24—48h显著下降，三者之间的差异有统计学意义（P均〈0．01）。换液后ET-1和SCF变化趋势基本同换液前（P均〈0．01）。M2中细胞增殖最快，角质形成细胞呈集落样生长，黑素细胞的树突较多。培养基1（M1）中贴壁细胞数目少，死亡细胞多，细胞增殖缓慢，角质形成细胞散在，黑素细胞多是双极。培养基3（M3）细胞生长情况介于M1和M2两组之间。结论培养基中的高水平ET．1有利于表皮细胞的贴壁、增殖和分化。在培养过程中表皮细胞能够分泌ET-1，并释放进入培养上清，表皮细胞培养消耗SCF，但无明显分泌作用。

英文摘要：

Objective To observe the growth of epidermal cells in three groups of media and detect the levels of ET-1 and SCF in the supernatants at different time points. Methods Three kinds of cell culture media were used to culture epidermal cells. The supernatants were collected at 24h, 48h, 72h and 96h after cells seeding, respectively. The levels of endothelin-1 （ET-1） and stem cell factors （SCF） in these supernatants were de- tected by the enzyme linked immunosorbent assay （ELISA） . Meanwhile, the cells growth in different media were observed and compared. Results Among supernatants of 3 groups, the content of ET-1 in medium 2 （M2）supernatants was the highest. All of the content of ET-1 were increased with prolonged culture time. The contents of SCF in the three groups of supernatants decreased significantly in 24 - 48h. There were sig- nificant difference among the three groups（P 〈0.01 ） for both ET-1 and SCF. After renewed the medium, the trends of ET-I and SCF were not pronounced （P 〈 O. 01 ）. The cell proliferation in M2 was fastest, where the keratinocytes grew in colony and the melanocytes displayed multiple dendrites. In medium 1 （M1）, less adherent cells, more death cells and slow cell proliferation were observed. The keratinocytes scattered in the medium 1, in which most of melanocytes showed bipolar. The growth of the cells in medium 3 （M3） was between that in M1 and M2. Conclusion High level of ET-1 in medium facilitates adhesion, proliferation and differentiation of epidermal cells. During the process of culture, the epidermal cells may se- crete and release ET -1 into supernatant. The epidermal ceils may do not secrete SCF but could digest it.