采用根袋土培试验研究了重金属Zn胁迫对黑麦草(Loliurn perenne L.)生长、抗氧化酶活性、游离脯氨酸含量、根系活力及锌积累的影响。结果表明,黑麦草对重金属锌有很强的抗性和耐性.即使在高锌下(16mmol Zn/kg)。黑麦草生长也未受到抑制。黑麦草地上部干重、根系千重在8mmol Zn/kg下达到最大值。分别较对照增加48.8%,52.2%;高锌处理(16mmol Zn/kg)的植株地上部千重也较对照增加11.2%。黑麦草POD活性随Zn胁迫增加,先下降,然后回升SOD活性则随锌胁迫增加而下降。随Zn胁迫增加,叶脯氨酸、根系活力先增加,然后下降。地上部、根系Zn含量随Zn胁迫增加而增加,最大值分别为746.5,521.0mg/kg DW(16mmol Zn/kg)。高锌处理下(≥4mmol/kg)Zn转运率(S/R)〉1.0。
Pot experiments (root rag method) were carried out to investigate the influence of Zn stress on plant growth, antioxidation enzyme activity, proline content, root system vigor, and Zn accumulation in ryegrass ( Loliurn perenne L. ). The results showed that the plants of ryegrass had high tolerance to Zn, even at higher Zn level (16 mmol/kg), inhibition to the plant growth was not observed. The maximums of shoot and root dry weights were recorded at 8 mmol/kg Zn increased by 48.8%, 52.2% contrast to the controls. Shoot dry weight was increased by 11.2% at high Zn treatment (16 mmol Zn/kg) . Shoot POD activities were first reduced, and then increased, while shoot SOD activities were reduced along with increasing Zn stress. Shoot proline contents and root system vigor were increased at first, and then decreased with increase of Zn stress. Zn contents in shoot and root were increased under Znstress, the maximums Zn contents in shoot and root were 746. 5, 521.0 mg/ kg DW(16mmol Zn/kg), respectively. Zn transport from roots to shoots (S/R) of plants was more over than 1.0 at higher Zn level ≥4 mmol Zn/kg).