Microsatellite genotyping 和技术经常是的放大碎片长度多型性(AFLP ) 在危及的动物的保存遗传的研究利用了。为保存危及的森林麝香鹿选择一个更有效的标记系统,我们使用了 microsatellite 和 AFLP 标记估计二张人口,纯母亲 Jinfengshan (JFS ) 组和后代的基因差异的层次有新血的介绍的 Baisha (BS ) 组。如果没有新鹿被介绍进 BS,这被假定, JFS 将比 BS 拥有显著地更高的基因可变性,这被期望如果它被假定。结果表明 15 个 microsatellite 标记生产了 141 等位基因和产出的 22 AFLP 教材联合 908 个多态的乐队,显示出那 AFLP 让更高的解决比 microsatellites 驱动。与另外的鹿相比,尽管如此,两个标记系统显示森林麝香鹿包含一基因变化相对高级。15 microsatellite loci 显示基因差异的类似的水平在 JFS 和 BS 人口是在场的,看起来建议新血显然在 BS 提高了基因差异的水平。然而, AFLP 标记的 22 间套房让步了一在 JFS 的基因变化显著地高级与比在 BS,与 microsatellites 的差异模式相对照。鉴于差别尽最大努力这二个标记系统,我们断定那基因基因渗入在 BS 人口是有限的,由 AFLP 标记揭示了。从整个染色体可变性的观点,而且,我们建议 AFLP 标记系统对森林麝香鹿上的保存遗传研究更合适。
Microsatellite genotyping and amplified fragment length polymorphism (AFLP) techniques are often utilized in studies of conservation genetics of endangered animals. To select a more effective marker system for conserving the endangered forest musk deer, we used microsatellite and AFLP markers to estimate levels of genetic diversity of two populations, the pure mother Jinfengshan (JFS) group and the offspring Baisha (BS) group with introduction of new blood. It was expected that JFS would pos- sess significantly higher genetic variability than BS if it is assumed that no new deer were introduced into BS. The results revealed that the 15 microsatellite markers produced 141 alleles and the 22 AFLP primer combinations yielded 908 polymorphic bands, showing that AFLPs had higher resolving power than rnicrosatellites. Nonetheless, compared with other deer, both marker systems indicated that the forest musk deer contains a relatively high level of genetic variation. The 15 microsatellite loci indicated a similar level of genetic diversity was present in the JFS and BS populations, seemingly suggesting that the new blood obvi- ously elevated the level of genetic diversity in BS. However, 22 suites of AFLP markers yielded a significantly higher level of genetic variation in JFS than that in BS, in contrast to the diversity pattern of microsatellites. In view of the difference in resolving power of these two marker systems, we conclude that gene introgression was limited in the BS population, as revealed by the AFLP markers. Furthermore, from the perspective of whole genome variability, we recommend that the AFLP marker system is more suitable for conservation genetics studies on the forest musk deer.