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中文摘要：
报道了一种采用谷氨酸席夫碱Ni（Ⅱ）配合物共保护三-谷氨酸α-氨基和α-羧基合成谷胱甘肽（y—L-谷氨酰正-半胱氨酰-甘氨酸）的新方法．首先由手性助剂——2-[N-（NO苄基-脯氨酰）氨基]二苯甲酮（1）、六水合氯化镍和L-谷氨酸反应，得到谷氨酸席夫碱Ni（II）配合物2，产率为98％；进而采用二异丙基碳二亚胺（DIC）／1-羟基苯并三氮唑（HOBt）复合缩合剂法与S-苄基L-半胱氨酸反应，得到譬苄基-γ-L-谷氨酰也-半胱氨酸席夫碱Ni（Ⅱ）配合物3，产率为90％；接着同样采用DIC／HOBt复合缩合剂法与甘氨酸反应，得到口苄基-γ—L-谷氨酰-L-半胱氨酰-甘氨酸席夫碱Ni（Ⅱ）配合物4，产率为95％；然后稀酸水解配合物4，得到墨苄基-γ-L-谷氨酰-L-半胱氨酰甘氨酸（5），产率为70％；最后采用甲酸铵催化转移氢化脱除S-苄基，得到谷胱甘肽（6），产率为87％．中间产物和终产物的结构经由旋光，1HNMR，^13CNMR和HRMS表征．
英文摘要：
A novel method for the synthesis of glutathione （γ-L-glutamyl-L-cysteinyl-glycine） via a Ni（Ⅱ） complex of glutamic acid Schiff base, which has been employed as co-protection of the α-amino and α-carboxyl groups of L-glutamic acid, was described. Firstly, a Ni（Ⅱ） complex 2 of glutamic acid Schiffbase was formed in a 98% yield from chiral auxiliary 2-[N-（N＇-benzyl-prolyl）amino]benzopheone （1） with nickel（Ⅱ） chloride hexahydrate and L-glutamic acid. Then, 2 reacted with S-benzyl-L-cysteine to give Ni（Ⅱ） complex 3 of S-benzyl-γ-L-glutamyl-L-cysteine Schiff base in a yield of 90% using a N,N＇diisopropylcarbodiimide （DIC）/1-hydroxybenzotrizole （HOBt） coupling method. Thirdly, 3 reacted with glycine to give Ni（Ⅱ） complex 4 of S-benzyl-γ-L-glutamyl-L-cysteinyl-glycine Schiff base in a yield of 95% using the same DIC/HOBt coupling method. Fourthly, after decomposition of 4 with aqueous HC1, S-benzyl-γ-L-glutamyl-L-cysteinyl-glycine （5） was obtained in a 70% yield. Finally, the S-benzyl was re- moved by catalytic transfer hydrogenation with ammonium formate, while glutathione （6） was synthesized in an 87% yield. Structures of intermediates and final products were characterized by optical rotation, 1H NMR, 13C NMR and HRMS techniques.
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