中文摘要：

酵母双杂交技术在拟南芥cDNA文库中筛选得到了与ABA受体蛋白RCARl，RCAR3均有相互作用的蛋白质VDAC3，并已获得VDAC3基因的全长．VDAC3蛋白包含有3个结构域，分别为Porin3结构域，DUF3442结构域和SEG片段，现根据结构域分布，将VDAC3截短为包含DUF3442的VDAC3N以及包含SEG的VDAC3C两段．将VDAC3全长以及分别截短的169个氨基酸（VDAC3N）及105个氨基酸（VDAC3C）的片段进行酵母转化实验，与RCARl或RCAR3共同转化后，VDAC3，VDAC3N的共转酵母能够在缺陷型培养基上生长，并且使X—Gal显色为蓝色，而VDAC3C则不能．这验证了全长的VDAC3与RCARl，RCAR3的存在相互作用，并发现决定VDAC3蛋白与RCARl，RCAR3是相互作用的结构域位于N端的DUF3442结构域．

英文摘要：

VDAC3 was screened out as interacting protein with abscisic acid（ABA） receptors RCAR1 and RCAR3 in Arabidopsis thialiana cDNA library by the yeast two hybrid system before. VDAC3 contains three domains. Porin3 domain,DUF3442 domain and SEG fragment. In this research VDAC3 was cut into two part, VDAC3 N which contains DUF3442 domain while VDAC3 C which contains SEG frag ment. Cotransform BD-RCAR1, BD-RCAR3 and full length AD-VDAC3, AD-VDAC3 N （507bp） into yeasts. The reconstructedyeasts can grow on themediumSD/-His/-Trp/-Leu/-Ade and turn blue in the presence of X-Gal while yeasts cotransformed BD-RCAR1, BD-RCAR3and AD-VDAC3 C（318 bp）can＇t. These experiments proved thatthe full length VDAC3 indeed interacts with RCAR1 and RCAR3,and al so N-termini DUF3442 domainpossesses the interactions.