中文摘要：

以水稻（Oryza sativa L.）品种Ⅱ优128种子为材料,利用激光扫描共聚焦显微技术及荧光标记等手段,解析萌发水稻种子糊粉层细胞程序性死亡（Programmed Cell Death,PCD）中液泡的形态变化,揭示液泡化对PCD进程的影响.结果表明：糊粉层细胞PCD过程液泡的形态结构会发生一系列变化,由最初较小的蛋白贮藏液泡,逐渐相互融合成为大的液泡,之后液泡破裂消失;液泡化现象是糊粉层PCD的显著特征,且其进程会直接影响到糊粉层PCD的进程;抑制液泡融合阻缓了糊粉层PCD进程,从而影响水稻种子的萌发.

英文摘要：

This study explored the vacuolization morphological variation of aleurone cells in germinating rice （Oryza sativa L. ） varieties ＇II 128＇ seeds during the process of PCD, and revealed the effect of vacuolization on the progress of PCD, by using confocal laser scanning microscopy and fluorescent labeling. The results showed that the morphological structure of vacuole changed a lot in the PCD of aleurone layers, the initial small protein-storage merging to a large vacuole, finally vacuoles brokening and disappearing. The vacuolization was a remarkable feature of PCD in aleurone layers. And its process would directly affect the PCD process of aleurone layer. Therefore, the inhibited vacuoles fusion delayed the course of aleurone layers PCD, thus affecting the germination of rice seeds.