中文摘要：

目的观察苯丙氨酸二肽类化合物073对2．2．15细胞分泌HBsAg、HBeAg的影响。方法以2．2．15细胞为研究对象，通过细胞毒性实验确定苯丙氨酸二肽类化合物073对细胞的最大无毒浓度（TC0），在最大无毒浓度以下将药物用培养液对倍稀释成50、25、12．5μg·mL^-1加入2．2．15细胞进行培养，每4d换同浓度药物培养液；分别收集第4、8天以及停药后4天的培养液上清，酶联免疫法（ELISA）测定HBsAg、HBeAg含量。结果苯丙氨酸二肽类化合物073最大无毒浓度为50μg·mL^-1，在最大无毒浓度时对HBsAg有明显的抑制作用，第8天时的抑制率为59．3％，半数抑制浓度（IC50）为22．9μg·mL^-1，治疗指数（TI）为3．1；但对HBeAg无明显抑制作用，第8天时的抑制率仅为25．5％。结论苯丙氨酸二肽类化合物073对2．2．15细胞HBsAg有显著的抑制作用。

英文摘要：

Aim To study the effect of phenylalanine dipeptide compounds 073 （ PDC073 ） on the secretion of HBsAg and HBeAg by 2.2.15 cells. Methods 2.2.15 cells were studied in the vitro model, and the maximum non-toxic concentration （ TC0 ） was identified by cell toxicity experiment of PDC073. Under the TC0, a pharmaceutical anti-virus experiment was performed. PDC073 was diluted by culture medium into different concentrations of 50, 25 and 12.5 μg·mL^-1 , which were added into the cell culture medium respectively. The medium old the same concentration was replaced every 4 days. On the 8th day the medium was changed to the drug-blank medium. The superuatant lipuid was collected on the 4th, 8th day and 4 days after treatment. ELISA method was used to assay the content of HBsAg and HBeAg. Results The maximum non-toxic concentration of PDC073 was 50μg·mL^-1. At this concentration of PDC073 the HBsAg was obviously IC50 was 22.9μg·mL^-1 and TI was 3.1. However, the inhibited. The inhibition ratio on the 8th day was 59.3% ; HBeAg was little inhibited, as the inhibition ratio was only 25.5% on the 8th day. Conclusion The PDC073 has significant inhibitive effect of HBsAg on cultured cell line 2.2. 15.