中文摘要：

目的探讨脂联素对体外高糖环境下培养的人近曲肾小管上皮细胞（HK-2细胞）凋亡及氧化应激水平的影响。方法将人近曲肾小管上皮细胞分为3组：正常对照组（5．5mmol／LD-葡萄糖）、高糖组（30．0mmol／LD-葡萄糖）、高糖＋脂联素组（30．0mmol／LD．葡萄糖＋2．5μg／ml脂联素），各组细胞分别培养24、48、72h后，采用流式细胞仪测定细胞凋亡率，黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性，硫代巴比妥酸法测定丙二醛（MDA）含量，实时荧光定量PCR测定细胞血红素加氧酶-1（HO-1）及衔接蛋白p66ShcmRNA表达水平。结果与正常对照组相比，高糖组细胞凋亡率增高，细胞培养液内SOD活性下降，MDA含量增高，同时诱导细胞HO．1及p66ShcmRNA表达上调，且呈时间依赖性（P〈0．05）。与高糖组相比，高糖＋脂联素组细胞凋亡受到抑制，细胞培养液内SOD活性增高，MDA含量降低，细胞HO-1mRNA表达进一步增强，而p66ShcmRNA表达降低，呈时间依赖性（P〈0．05）。结论随时间延长，高糖环境可引起HK-2细胞过度凋亡和高水平氧化应激；脂联素可通过延缓细胞凋亡和抑制氧化应激来发挥对’肾脏的保护作用。

英文摘要：

Objective To investigate the effects of adiponectin on apoptosis and oxidative stress in human renal tubular epithelial cells cultured in high concentration of D-glucose in vitro. Methods The human renal tubular epithelial cells were divided into 3 groups： normal control group （5.5mmol/L D-glucose）, high glucose group （30.Ommol/L D-glucose） and high glucose plus adiponectin group （30.0mmol/L D-glucose＋2.5μg/ml adiponectin）. After being cultured for 24, 48, 72h, flow cytometry were employed to determine cellular apoptosis. Superoxide dismutase （SOD） and malondialdehyde （MDA） in the supernatant of culture media were determined with xanthine oxidase method and thiobarbituric acid method. Heine oxygenase-1 （HO-1） mRNA and adaptor protein p66Shc mRNA expression were determined by RT-PCR. Results Compared with normal control group, high glucose could induce apoptosis, lower SOD activity and elevate MDA level （P〈0.05）. The mRNA expression of HO-1 and p66Shc were both increased in high glucose group time dependently （P〈0.05）. Compared with high glucose group, addition of adiponectin could inhibit apoptosis, increase SOD activity and lower MDA level （P〈0.0S）. The expression of p66Shc mRNA decreased but HO-1 mRNA continued to increase in a time dependent manner （P〈0.05）. Conclusions High D-glucose content could induce HK-2 cells apoptosis and oxidative stress. Adiponectin could deter apoptosis and inhibit oxidative stress to exert a protective effect on kidney.