目的:分析秦岭地区野生华中五味子的遗传多样性。方法:应用SSR分子标记进行遗传多样性分析。结果:9对SSR引物共检测到61条条带,每对引物扩增出5~11条,平均每对引物可扩增出6.8条,多态性比率为86.88%。遗传分化系数(Gst)为0.3524,表明秦岭地区华中五味子遗传变异主要存在于居群内,基因流(Nm)为1.1850,说明居群间存在一定基因流。通过遗传多样性参数比较,洋县居群遗传多样性最高。聚类分析可划分为2大类,第1类包括平利县、山阳县和宁强县居群;第Ⅱ类包括太白县和洋县居群。结论:秦岭地区华中五味子遗传多样性丰富,应优先选择陕西汉中市、宝鸡市和商洛市地区华中五味子居群实行就地保护与扩繁,以保证野生华中五味子可持续发展和利用。
Objective:To analyse the genetic diversity of wild Schisandra sphenanthera in Qinling Mountains. Methods:The genetic diversity of wild S. sphenanthera was investigated by SSR ( Simple Sequences Repeats) markers. Results : Nine pair primers generated a total of 61 bands 53 of which were polymorphic, and 6. 8 genotypes were detected by each SSR primer pair on an average ranging from 5 to 11. The percentage of polymorphic band was 86. 88 %. The coefficient of genetic differentiation ( G~, = 0. 3524) showed great genet- ic variation occurred within populations. Gene flow (Nm ) was 1. 1850, which showed that gene flow occurred among populations. The highest genetic diversity of five populations was Yangxian population. And cluster analysis was based on genetic similarity coeffi- cient. The five populations of wild S. sphenanthera samples were classified into two large groups, group I included Pingli population, Shanyang population and Ningqiang population; Group II included Taibai population and Yangxian population. Conclusion : The S. sphenanthera in Qinling Mountains, especially the regions of Hanzhong, Baoji and Shangluo in Shaanxi province, reveals high genetic di- versity, which benefits the breeding of new varieties. It is necessary to protect the resource and ensure the sustainable development and utilization of it.