中文摘要：

目的：为进一步研究PDX-1基因的功能打下基础。方法：从人的胰腺cDNA中PCR扩增PDX-1基因的阅读框架,构建质粒pAAV-PDX-1。用pAAV-PDX-1转染293T细胞48小时后,用RT-PCR和Western Blot方法检测293T细胞中mRNA和蛋白水平PDX-1的表达。结果：转染了pAAV-PDX-1的293T细胞中有PDX-1的表达,而在转染质粒pAAV-MCS和未转染的293T细胞中均没有检测到PDX-1的表达。结论：构建的pAAV-PDX-1质粒在mRNA和蛋白水平都能表达PDX-1。

英文摘要：

Objective：To further study the function of the PDX-1 gene. Methods： We directly cloned a 987-bp fragment including PDX-1 open reading frame （ORF） from the human pancreas cDNA to construct pAAV-PDX-1 plasmid. The plasmid was transfected into the 293T cells and the expression of PDX-1 was analyzed by RT-PCR and Western blot analysis. Results： The PDX-1 expression was detected in the 293T cells transfected with pAAV-PDX-1 while not detected in the 293T cells transfected with control plasmid or untransfected. Conclusion： We successfully constructed plasmid pAAV-PDX-1 that expressed the PDX-1, which provide the base to study the function of the PDX-1.