中文摘要：

目的观察人偏肺病毒（human metapneumovirus，hMPV）A和B亚型感染BALB／c小鼠后，小鼠在体重、病毒载量、病理及气道反应性等方面的改变，探讨两亚型病毒致病性上的差异，为hMPV的进一步研究提供基础资料。方法hMPV感染BALB／c动物模型后，通过使用real—timeRT—PCR方法检测鼠肺内病毒载量的变化，肺功能检测仪监测小鼠气道反应性的变化及组织系统评分判定病理改变情况，来观测两亚型致病性的差异。结果hMPV两亚型感染组，在体重的动态监测、病毒载量、肺组织病理改变及气道反应性上差异均无统计学意义。结论hMPV两基因型感染BALB／c小鼠的致病性无差异。

英文摘要：

Objective To investigate the difference of pathogenicity between the two genotypes of human metapneumovirus （hMPV） for the further research. Methods At various time after hMPV infection in BALB/c mice, viral titers of lung tissue were measured by real-time RT-PCR, pathology was assessed by a histopathological scoring system, airway responsiveness was assayed by animal lung function monitoring equipment . Pathogenicity was then measured by detailed evaluation through the results above . Results There is no significant difference in weight of mice between control group and experimental group through dynamic monitoring; though the difference was exists in airway responsiveness and pathological changes of mice between control group and experimental group, the differences were not statistically in airway reaction, path- ological changes and virus drops among the three groups of experimental group. Conclusion There is no difference in pathogenicity between the two subtypes of hMPV in infection of BALB/c mice, viral genotype do not appear to be associated with pathogenicity.