中文摘要：

传统的三维支架细胞培养技术已能较好地实现细胞在培养过程中的空间排布,但是其结构仍不具有类血管网络的通道结构,尚不能完全模仿人体内部的细胞生长环境。在3D打印生物支架的基础上,借助强制结构优化方法,利用VS平台上的C＋＋自编译软件,在三维生物支架STL模型内部构建出模拟的三维血管通路,生成新的三维生物支架STL模型,并利用3D生物打印机打印出实物。通过对打印支架的空隙和血管大小的统计分析以及与数字模型的参数对比分析得出,打印出的实物支架孔隙（孔隙间距、线宽）的平均值及打印血管（孔隙间距、线宽）的平均数据与对应数字模型的设定数据的误差均在5%以内,符合设计要求。另外,该研究可增加细胞培养支架内部液体的流通性,使支架结构进一步接近人体内环境,为模拟人体内液体循环对于细胞生长的影响提供可能。

英文摘要：

The three-dimensional scaffold cell culture technology has achieved a good spatial structure in the process of cell cultivation; however there are still lack of channel structures such as vascular network in the 3D scaffolds. Therefore current 3D scaffolds can not entirely simulate the growing environment of internal cells of the human body. Referring to the method of constrained structure optimization,this article built a simulated three-dimensional vascular network that was constructed inside the STL model of biological scaffold by computer software on the basis of common three-dimensional biological scaffold and created a new kind of threedimensional biological scaffold model and printed the material objects using 3D biological printer. Through the statistical analysis of scaffold model and compared with the digital model,the differences between the printed scaffold model and the digital model were within 5%,which met the design requirements. By this technique the liquid circulation inside the cell culture scaffold was increased and the structure of scaffold was closer to the internal environment of the human body. The research can also make it possible to simulate and figure out factors of affecting the liquid circulation inside the human body has on the growth of cells.