中文摘要：

研究了一种新型丙烯酸酯（E）-2-氰基-3-[4-（2,4-二氟苯氧基）-苄氨基]-3-甲氧基-丙烯酸2-乙氧基乙酯（MAE）对超螺旋pUC19 DNA的定点切割作用。利用紫外吸收、荧光光谱、凝胶电泳和DNA测序技术研究其核酸切割特异性。紫外吸收光谱的红移和减色效应,以及静态荧光猝灭表明MAE与DNA的作用属于典型的插入模式。不仅如此,凝胶电泳条带的变化以及DNA测序结果表明DNA的定点切割是通过DNA双链的分步水解完成。

英文摘要：

Here a novel cyanoacrylic ester,（ E）-2-cyano-3-[4-（ 2,4-difluorophenoxy） benzylamino]-3-methoxyacrylic acid 2-ethoxyethyl ester（ MAE）,which is capable of site-specific cleavage of supercoiled pUC19 DNA,was introduced. The cleavage specificity was investigated through UV absorption,fluorescence,agarose gel electrophoresis,and DNA sequencing techniques. Red shift and hypochromism in the absorption spectra and static fluorescence quenching suggested that a typical intercalation occurred between DNA and MAE. In addition,detectable varieties in the gel electrophoresis and DNA sequencing indicated that the cleavage was site-specific,and double strands of DNA were hydrolyzed step by step.