中文摘要：

目的：探讨外源性降钙素基因相关肽（CGRP）对体外培养兔成骨细胞一氧化氮／一氧化氮合成酶（NO/NOS）系统的时序调控作用。方法：将体外培养的成骨细胞用不同浓度的CGRP处理0．5h、1h、4h和12h，通过Western—blot检测细胞NOS的蛋白表达变化，使用NOS分型试剂盒测定细胞NOS的酶活性，硝酸还原酶法检测细胞培养液中NO浓度。结果：在0．5h－12h时间段，CGRP实验组细胞培养液中NO含量明显高于对照组（p〈0．05或p〈0．01）；实验组成骨细胞中eNOS的表达和酶活力明显高于对照组（p〈0．05或p〈0．01），而iNOS的表达和酶活力检测未发现有明确的统计学差别。结论：CGRP可通过调节成骨细胞的N0／NOS系统促进成骨细胞的增殖分化从而影响骨代谢。

英文摘要：

Objective： To study the regulation of extrinsic calcitonin gene-related peptide （CGRP） on the NO/NOS system of rabbit osteoblasts in vitro. Methods： Osteoblasts were cultured in cultural medium containing CGRP in different concentration. After 0.5, 1, 4 and 12 hours of incubation, the expression of NOS protein were examined by Western-biottin~g, the enzymatic activity of NOS were determined with the NOS detecting Kit, and the concentration of NO in the cell suspension were determined by nitrate reducase method. Results： Compared with the control group, Concentration of NO significantly increased in the cell suspension after the treatment of CGRP with 0.5h -12h （p〈0.05 or p〈0.01 ）, the expression and enzymatic activity of eNOS in osteoblasts was higher in the treated groups than in the control group （p〈0：05 or p〈0.01 ）. There were no statistical difference in the expression and enzymatic activity of iNOS at each time point. Conclusions： CGRP promotes the proliferation and differentiation of osteoblasts by regulating the NO/NOS system in osteoblasts.