中文摘要：

目的研究川芎嗪注射液对铅中毒小鼠学习记忆功能的影响。方法将50只健康4周龄清洁级昆明小鼠按体重随机分为5组，分别为空白对照（生理盐水）组、铅中毒组、阳性对照组和低剂量川芎嗪治疗组、高剂量川芎嗪治疗组，每组10只。除空白组外，其他4组腹腔注射70mg／kg乙酸铅溶液，染毒容量为14ml／kg，每日1次，连续注射8d。铅中毒模型复制完成后恢复3d，空白组和铅中毒组均连续7d腹腔注射生理盐水；低、高剂量川芎嗪治疗组分别连续7d腹腔注射40、80mg／kg川芎嗪溶液；阳性对照组前3d腹腔注射80mg／kg依地酸钙钠溶液，后4d腹腔注射等量生理盐水；注射容量为20ml／kg，每日1次。采用Morris水迷宫法检测小鼠学习记忆能力，采用生物化学法检测小鼠大脑组织中乙酰胆碱转移酶（ChAT）及乙酰胆碱酯酶（TChE）的活力，并计算海马细胞坏死率。结果与空白组相比，铅中毒组小鼠逃避潜伏期明显延长，海马细胞坏死率和脑组织中TchE活力明显升高，脑组织中ChAT活力显著降低，差异有统计学意义（P〈0．05）。与铅中毒组相比，高剂量川芎嗪治疗组小鼠逃避潜伏期明显缩短，海马细胞坏死率明显下降，脑组织中ChAT活力显著提高，而各剂量川芎嗪治疗组小鼠脑组织中TchE活力明显降低，差异有统计学意义（P〈0．05）。且随着川芎嗪溶液染毒剂量的升高，铅中毒小鼠海马细胞坏死率和脑组织中TchE活力呈下降趋势，脑组织中ChAT活力呈上升趋势，逃避潜伏期呈缩短趋势。结论川芎嗪注射液能改善铅中毒小鼠的学习记忆能力，其作用机制可能与其降低小鼠脑组织中TchE活力、提高ChAT活力，增强中枢胆碱能神经系统功能有关。

英文摘要：

Objective To study the influence of ligustrazine on learning and memory ability on lead poisoning mice. Methods Fifty mice （four weeks old） were randomly divided into five groups based on body weight, 10 in each, blank group, lead poisoning group, positive control group, low dose group and high dose group of ligustrazine treatment. By intraperitoneal injection, the mice in the blank group were given sallne,the mice in the other groups were given 2% Pb（Ac）2 solution（70 mg/kg body weight） for eight consecutive days, followed by three days of recovery. Then the mice in the blank group and lead poisoning group were still given saline for seven days, the mice in low dose group and high dose group of ligustrazine treatment were given ligustrazine （40 and 80 mg/kg body weight） through intraperitoneal injection for seven consecutive days. The mice in positive control group were given intraperitoneal injection of calcium disodium edetate （80 mg/kg body night） for three consecutive days, and the other four days the mice were given intraperitoneal injection of saline. Morris water maze was used to monitor the learning and memory ability. In the mean time,the activities of ChAT and TchE were measured by biochemical methods. And the neorobiosis rate in hippocampus was calculated. Results Compared with blank group, the escaping latency period of mice in lead poisoning group was extended obviously. The neorobiosis rates in hippocampus and TchE activity in brain tissue increased obviously, the ChAT activity decreased obviously（P〈0.05 ）. Compared with lead poisoning group, the above changed indexes were well recovered in low dose ligustrazine-treated group, the ChAT activity in low dose group and high dose ligustrazine-treated groups decreased significantly （P〈0.05）. As the dose of ligustrazine increased, the neorobiosis rate in hippocampus and TchE activity in brain tissue showed decreasing tendency, the ChAT activity in brain tissue showed increasibg tendency, and the escaping latency period show