中文摘要：

背景与目的：mi R-124在多种肿瘤中发挥抑癌基因样功能,如肺癌、前列腺癌、膀胱癌和乳腺癌,但是其在胃癌中的表达及临床意义尚不清楚。该研究旨在研究mi R-124在正常胃黏膜上皮细胞与不同胃癌细胞及胃癌组织及正常胃黏膜组织中的表达,分析其表达与胃癌患者性别、年龄、组织学分级、T分期、TNM分期、淋巴结转移及预后之间的相关性。方法：采用实时荧光定量聚合酶链反应（real-time fluorescent quantitative polymerase chain-reaction,RTFQ-PCR）检测mi R-124在人胃黏膜上皮细胞及胃癌细胞中的表达,采用原位杂交法检测mi R-124在胃癌及癌旁正常组织中的表达。结果：RTFQ-PCR结果显示,mi R-124在胃癌MKN-74、MKN-28、MKN-45、MGC-803、SGC-7901及AGS细胞中的表达均低于GES-1细胞;原位杂交实验结果显示,mi R-124在正常胃黏膜中呈强阳性表达,在胃腺癌组织中表达下降、局灶阳性或缺失;统计分析显示,mi R-124与胃腺癌患者组织学分级、TNM分期及淋巴结转移密切相关,与患者的年龄、性别及肿瘤大小无关;Kaplan-Meier生存曲线统计分析显示,mi R-124低表达患者的总生存时间和无病生存时间显明低于mi R-124高表达患者;多因素分析结果提示,mi R-124表达下调是影响患者生存的独立预后因素。结论：mi R-124在胃癌细胞及组织中表达下调,并且与患者的组织学分级、TNM分期、淋巴结转移及预后密切相关。

英文摘要：

Background and purpose： miR-124 is considered to be a tumor suppressor in multiple tumors, including lung cancer, prostate cancer, bladder cancer, and breast cancer. However, its function is unclear in gastric cancer. This study aimed to explore the expression of miR-124 in human gastric mucosal epithelium cells and different gastric cancer cells, as well as gastric cancer tissues and matched para-cancerous tissues. The correlations of miR-124 expression with gender, age, histological grade, T stage, TNM stage, lymph node metastasis and prognosis of gastric cancer patients were analyzed. Methods： A real-time fluorescent quantitative polymerase chain reaction （RTFQ-PCR） was employed for detecting the expression of miR-124 in human gastric mucosal epithelium cells and gastric cancer cells. The expression of miR-124 in gastric cancer tissues and matched para-cancerous tissues was detected by in situ hybridization. Results： The RTFQ-PCR results indicated that the expression of miR-124 was down-regulated in MKN- 74, MKN-28, MKN-45, MGC-803, SGC-7901 and AGS cells compared to GES-1 cells. In situ hybridization showed that miR-124 was strongly expressed in normal gastric mucosa. However, low expression, focal positive expression or lack ofmiR-124 expression were observed in gastric cancer tissues. Statistical analysis showed that miR-124 was close- ly correlated to histological stage, TNM stage and node metastasis of gastric cancer patients, but not the age, gender and tumor size. The OS and DFS of the patients with low expression of miR-124 were shorter than that of the patients with high expression of miR-124. Multivariate analysis suggested that miR-124 down-regulation was an independent prognostic factor for survival in patients with gastric cancer. Conclusion： miR-124 is down-regulated in gastric cancer cells and tissues. The expression ofmiR-124 is correlated to histological stage, TNM stage, node metastasis and prognosis.