研究了中华鲟精子和卵子在低温(8,12℃)、中温(16,20℃)和高温(24,28℃)条件下短期保存的效果。结果发现:精子保存时间随着保存温度的升高逐渐缩短,8℃下可保存146h以上,而28℃下只能保存32h。低温下保存精子的活力强于高温下保存的精子,表现为快速运动时间和总活动时间较长;卵子适合在中温(16,20℃)下保存,高温或者低温对卵子的保存都不利,28℃下只能保存4-8h,8℃下只能保存4-10h,而在16℃和20℃下可保存18-20h。
The effect of low temperature (8, 12 ℃ ), medium temperature ( 16, 20 ℃ ) and high temperature (24, 28 ℃ ) on short-term preservation of Chinese sturgeon sperm and egg was studied for the first time. The result showed: Along with the temperature rising, the preservation time of sperm reduced. Sperm could be preserved under 8 ℃ for above 146 h, compared with only 32 h under 28 ℃. The motility of sperm preserved under low temperature (8, 12 ℃ ) was stronger than the others, and had longer quick-movementtime and total-movementtime. The egg was suitable to be preserved under medium temperature ( 16, 20 ℃ ), while high temperature or low temperature was all disadvantageous for egg preservation. Egg could only be preserved for 4-8 h under 28℃, only 4 - 10 h under 8℃, however, 18 - 20 h under 16℃ and 20℃.