本研究以广泛分布于长江、珠江等水系的经济鱼类瓦氏黄颡鱼( Pelteobagrus vachelli Richardson)幼鱼为实验对象,选取实验鱼42尾,体重和体长分别为(13.10±0.59)g、(9.63±0.15)cm;在(25±1)℃的条件下,分别测定了其静止状态和力竭性运动后恢复时间为0、1、2、4、8和16h时的肌肉乳酸含量和血糖浓度。结果显示,瓦氏黄颡鱼幼鱼静止状态时肌乳酸浓度为(5.16±0.34)μmoL/g;力竭性运动后肌乳酸迅速上升,并在运动后恢复0h达到峰值(16.11±1.06)μmol/g,经计算其变化倍率约为3.1;随后逐渐下降,恢复4h后与静止水平无显著性差异,乳酸清除速率为2.4μmol·h^-1·g^-1;瓦氏黄颡鱼幼鱼恢复过程中肌乳酸含量随时间变化的拟合曲线方程为Y=5.2199±11.2333e^-0.4366x(序=0.8395,P〈0.001,n=36)。血糖浓度呈现先下降后上升随后又下降的变化趋势,运动后在恢复0h时显著下降并达到(4.31±0.48)mmol/L这一最低水平(p〈0.05)。经与其它几种鱼类的相关资料比较,本研究提示力竭运动后瓦氏黄颡鱼幼鱼的肌乳酸峰值水平较低、恢复时间较短;推测该实验鱼的无氧代谢能力可能较弱,但短期内具有较强的重复运动能力。
Juvenile darkbarbel catfish (Pelteobagrus vachelli Richardson), an economic fish species distributed widely in the Yangtze River and Pearl River, are chosen as the research animal. The body weight and body length of total 42 experimental fish are (13.10 ± 0.59) g and (9.63 ±0.15 ) cm, respectively. Muscle lactate and blood glucose levels of juvenile darkbarbel catfish are measured at rest (resting controls) and during different recovery periods (0, 1,2, 4, 8, 16 h) after exhaustive exercise at (25 ± 1 ) ℃ in the study. The results reveal: the muscle lactate levels of juvenile darkbarbel catfish were (5.16 ± 0.34) μmol/g at rest (resting controls), then it increased rapidly after exhaustive exercise, and peaked immediately (recovery 0 h) at (16.11 ± 1.06) μmol/g, then gradually decreased. It returned to resting levels within 8.4 h of recovery and its lactate clearance rate (LCR) was 1.3μmol/( h· g). The lactate factorial scope (LFS) evoked by exhaustive exercise in the present study was about 3.1. The relationship between muscle lactate concentrations and resume time after exhaustive exercise can be described as the following equation: Y = 5. 219 9 + 11. 233 3e^-0.4366x (R^2 = 0. 839 5, p 〈0. 001, n = 36). The blood glucose concentration of juvenile darkbarbel catfish showed a decrease-increase-decrease course after exhaustive exercise. The lowest value was presented immediately (recovery 0 h) after exhaustive exercise ( (4.31 _± 0.48 ) retool/L, p 〈 0.05 ) , after it increased slowly to a level similar to that of resting controls (p 〉 0.05 ). The results showed that: the low muscle lactate level and fast recovery rate after exhaustive exercise of juvenile darkbarbel catfish in the present study have suggested that this fish has a weak anaerobic metabolism but a strong short-term repeat movement capacity.