抗生素对细菌具有强抑制作用,从而会影响废水生物处理系统中的微生物群落结构。在用定量PCR方法对土霉素生产废水处理装置(进水中土霉素浓度为1 662.1±248.6μg/L)中的细菌16S rRNA基因和真菌18S rRNA基因的含量进行比较的基础上,利用16S rRNA基因克隆文库方法对污泥中的细菌群落结构进行了详细解析。定量PCR结果显示,土霉素废水活性污泥中真菌(18S rRNA)/细菌(16S rRNA)基因的拷贝数比例高达1.2,明显较高于非抗生素肌苷废水活性污泥中的比例1.52×10-6,表明真菌对于土霉素废水中有机物的去除可能发挥重要作用。细菌克隆文库分析结果显示,Alpha变形菌和Beta变形菌是主要的优势菌,比例分别为23.7%和22.0%,其次是酸杆菌(17.0%)和拟杆菌(11.9%)。
As antibiotics can cause strong inhibition of bacteria,their presence will influence microbial community structure in wastewater treatment plants.The abundances of bacterial and fungal populations in an oxytetracycline-production wastewater(influent oxytetracycline 1 662.1±248.6 μg/L) were measured by quantitative real-time PCR,and bacterial community structure was examined by 16S rDNA clone library construction.The ratio of fungi/bacteria valued 1.2 in oxytetracycline-production wastewater,much higher than that(1.52×10-6) in inosine-production wastewater with no antibiotic.The results indicate that fungi may play an important role in COD removal of oxytetracycline-production wastewater treatment.Bacterial community structure analysis shows that Alphaproteobacteria(23.7%) and Betaproteobacteria(22.0%) are predominant groups,and then Acidobacteria(17.0%) and Bacteroidetes(11.9%).