中文摘要：

为满足现代光镊需要对捕获粒子进行高质量成像及特征参数分析的要求,设计并实现了一套光镊的成像系统,并对该系统硬件部分和软件部分进行了研究。首先,搭建了光镊系统,该系统主要由两部分组成,即粒子捕获系统和粒子成像系统。随后,基于电控精密三维平移台和可调焦成像物镜实现了捕获细胞的高质量成像;并基于OpenCV数据库开发了细胞特征参数分析系统,获得了任意感兴趣区域的捕获细胞数量、位置、形状及圆度等参数。最后,实现了酵母菌细胞成像和特征参数的分析和测试。实验结果表明：可准确实现细胞成像及特征参数分析,细胞位置精度达到1个CCD像素宽度,圆度分析精度达到0.01。基本满足了现代光镊对捕获粒子高质量成像及分析的要求,解决了捕获区域细胞粘连的难题,为进一步实现粒子力学特性研究提供了坚实基础。

英文摘要：

To meet the requirements of high-quality imaging and characteristic parameters analysis of trapped particles, an imaging system in an optical tweezer is designed and realized, including the hardware section and the software section. First, an optical tweezer is built, which mainly consists of a system to trap particles and a system to image and analyze trapped particles. Then, high - quality imaging of trapped particles is realized based on manipulations of an electric control 3D precise stage and a zoom objective lens. Meanwhile, a characteristic parameters analysis system of imaged particles is developed based on OpenCV, and numbers, position coordinates, shape parameters and circularity of trapped cells in the region of interesting are attained based on the system. Finally, the imaging and parameters measurement of yeast cells are carried out. Experimental results verify the feasibility and efficiency of the designed imaging system; the positioning precision of the trapped cell is one pixel of the CCD camera, and the measuring precision of cell circularity is O. 01. It can satisfy the system requirements of high-quality imaging and real-time characteristic parameters analysis for modem optical tweezers, solve the difficulty of characteristic parameters analysis of adhesive cells, and pave the way of mechanical properties study in future.