中文摘要：

利用自由基聚合反应将甲基丙烯酸聚乙二醇酯（PEGMA）和甲基丙烯酸缩水甘油酯（GMA）的二元共聚物接枝在基材表面,并通过开环反应分别固定精氨酸-甘氨酸-天冬氨酸（RGD）、精氨酸-谷氨酸-天冬氨酸-缬氨酸（REDV）和酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸（YIGSR）3种可特异性黏附内皮细胞的多肽.通过核磁共振检测合成的聚合物分子结构,并进一步通过X射线光电子能谱（XPS））以及原子力显微镜（AFM）的测试结果证明聚合物成功接枝在基材表面.利用紫外-可见吸收光谱（UV-Vis）对表面固定的3种多肽进行了定量表征.体外内皮细胞和平滑肌细胞黏附结果表明,3种不同多肽修饰的共聚物表面均能够有效阻抗平滑肌细胞的黏附,同时不同程度地促进内皮细胞的黏附,从而实现了基材表面内皮细胞的选择性黏附.其中与RGD和YIGSR多肽修饰的表面相比,REDV多肽修饰的表面呈现出更优异的内皮细胞选择性.这种具有内皮细胞特异选择性的界面在心血管支架涂层原位内皮化方面具有良好的应用前景.

英文摘要：

This paper aimed to describe a bioactive polymer biointerface for specifical of recognition endothelial cells. In particular,a random copolymer,poly（ ethylene glycol） methacrylate（ PEGMA） and glycidyl methacrylate（ GMA）（ PEGMA-GMA） was synthesized using AIBN as an initiator and grafted on PET substrate by solvent evaporation method. PEGMA unit could reduce nonspecific adsorption of platelet,protein and cells. GMA unit contains reactive epoxide functional groups on the side chains is beneficial to immobilization of antibody or peptide. The peptide sequence Arg-Gly-Asp（ RGD）,Arg-Glu-Asp-Val（ REDV）and Tyr-Ile-Gly-Ser-Arg（ YIGSR） were known to bind preferentially endothelial cells. As a result,endothelial specific peptide（ RGD,REDV and YIGSR） was conjugated through the epoxy groups in GMA segments.~1HNMR was used to characterize the composition of the polymer. Surfaces were characterized by X-ray photoelectron spectroscopy（ XPS） and atomic force microscopy（ AFM） to confirm the modification reactions.UV spectrum was used to calculate the content of peptide. Cell adhesion experiments showed that peptide modified surfaces inhibit the adhesion of smooth muscle cells（ SMCs） and promote endothelial cells（ ECs）growth,exhibiting excellent specific recognition of ECs on biomimetic coatings. Comparing with polymer coating modified by RGD and YIGSR,REDV conjugated surface exhibited the highest selectivity for ECs. The multifunctional polymer coating with anti-fouling and ECs specific adhesion produced an excellent prospect in cardiovascular stent implantation.