中文摘要：

目的探讨兔肝VX2瘤化疗栓塞介入术前后增殖细胞抗原（PCNA）、凋亡活化基因（Bax）、转移抑制基因23（nm23）、上皮型钙依赖黏连蛋白（E-cad）表达与扩散加权成像（DWI）表观扩散系数（ADC）值间的关系。方法40个兔肝VX2瘤模型，分为对照组与介入术后16、32、48h组，每组10个模型。4组分别在化疗栓塞介入术前与化疗栓塞介入术后16、32、48h行DWI，并对各组肿瘤标本行病理及免疫组织化学检查。对上述不同时间及不同部位（肿瘤周围正常肝组织、肿瘤外周部分组织、肿瘤周围部分组织、肿瘤中央部分组织）之间PCNA、Bax、nm23、E-cad表达指数以及表达指数与相应部位ADC值的关系进行分析。结果（1）对照组VX2瘤外周部分、周围部分与中央部分组织PCNA表达指数（分别为65．1％、74．7％、59．0％）明显高于周围正常肝实质（8．3％）（X^2=19．08，P〈0．01）；上述部位的nm23（分别为1．7％、0．4％、6．2％）、Bax（分别为2．0％、1．2％、2．2％）及E-cad（分别为6．2％、2．0％、1．6％）表达指数明显低于周围正常肝实质（分别为16．5％、40．0％、78．0％）（X^2值分别为12．86、20．17、22．20，P值均〈0．01）。（2）介入术后16、32、48h组VX2瘤周围部分组织PCNA表达指数分别为83．0％、92．6％、85．7％，nm23表达指数分别为2．3％、7．4％、4．2％，Bax表达指数分别为0．8％、0．5％、0．9％，E-cad表达指数分别为2．8％、1．0％、1．1％，与对照组上述指标比较，PCNA、nm23表达指数先增加后下降（x。值分别为14．37、8．94，P值〈0．05），Bax、E-cad表达指数差异无统计学意义（X。值分别为1．98、3．88，P值〉0．05）。（3）b=100s／mm。时，对照组与介入术后16、32、48h组肿瘤周围部分ADC值分别为（1．71±0．27）×10^-3、（1．24±0．22）×10^-3、（1．48±0．37）×10^-3及（1?

英文摘要：

Objective To investigate the correlation between the apparent diffusion coefficient （ADC） values and the expression of proliferating cell nulear antigen （PCNA）, Bax, non-metastasis 23（nm23） and E-cadherin （E-cad） genes in rabbit models of liver VX2 tumor before and after chemoembolization. Methods Forty rabbit models of liver VX2 tumor were divided into four groups with 10 rabbits in each group. The first group was the control group which didn＇t undergo chemoembolization. The second, third and fourth groups underwent chemoembolization, and diffusion weighted imaging（DWI） was performed at 16 h, 32 h and 48 h after chemoembolization respectively. The pathological and immunohistological examinations were carried out right after DWI. The sampling areas included the normal liver parenchyma around the tumor, the outer- layer area, the peripheral area, and the central area. The expression indices of PCNA, Bax, nm23, E-cad in all the samples were recorded and their correlation with corresponding ADC value were analyzed. Results （1） PCNA expression indices in the outer layer area,the peripheral area and central area of VX2 tumors（ 65.1% , 74.7% , and 59.0% respectively） were higher than that in the area of normal parenchyma around tumor（8.3% ） （ X2 = 19. 08, P 〈0. 01 ）. The nm23, Bax or E-cad expression indices in the the outer layer area, the peripheral area and the central area of VX2 tumors（ nm 23： 1.7% , 0. 4% , and 6. 2% respectively; Bax： 2.0% , 1.2% , and 2.2% respectively; E-cad：6. 2% ,2. 0%, and 1.6% respectively） were lower than that in the area of normal parenchyma around tumor （ nm23 16.5% ; Bax 40.0% ; E-cad 78.0%. X^2 = 12.86, 20. 17, and 22. 20 respectively; P 〈0. 01 ）. （2） The PCNA expression indices in the area of VX2 tumor periphery were 83.0% , 92. 6% and 85.7% in 16 h group, 32 h group and 48 h group respectively after chemoembolization and those of nm23 expression indices were 2. 3% , 7. 4% , 4. 2% and those of Bax expression index were 0. 8%