中文摘要：

在考察了国内外内源微生物采油技术研究情况的基础上，采用1#-4#激活剂配方对沾3区块注入水中微生物群落进行了选择性激活，对激活后总菌、有益菌、有害菌的数量及表面张力进行了检测，并进行了室内模拟驱油实验。实验结果表明，1#-4#激活剂配方能有效刺激有益菌的生长，同时对有害菌SRB实现了较为有效的抑制，压力和多孔介质对微生物的生长会产生一定的影响，多孔介质的影响较压力的影响要显著一些，KNO3的激活效果及提高残余油采收率的效果要优于NH4NO3，硝酸盐含量从0．1％到0．2％的增加能增加有益菌的数目，但对采收率的提高作用并不明显。室内模拟驱油实验表明，在水驱基础上内源微生物驱可提高采收率达6％以上。

英文摘要：

On the basis of summarizing the researches on indigenous microbial enhanced oil recovery at home and abroad, four activating nutrients were used to activate the indigenous microorganisms selectively. The total number of all bacteria, the numbers of beneficial bacteria and harmful bacteria and the surface tension of Zhan 3 injection water after activation were tested. Then, the physical simulation experiments of indigenous microbial flooding were performed. The results showed that these four activating nutrients could activate the beneficial bacteria successfully; meanwhile, the harmful bacteria SRB were restrained to some degree. Pressure and porous media could influence the growth of microorganisms, especially porous media. KNO3 was better than NH4NO3 in the effect of activation and enhancement of oil recovery. With increasing content of nitrate from 0. 1% to 0.2 %, the change of activation effect was very little. Core flooding experiments indicated that the indigenous microorganisms could improve residual oil recovery by above 6% after water flooding.