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Gold nanoprisms as a hybrid in vivo cancer theranostic platform for in situ photoacoustic imaging, angiography, and localized hyperthermia

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分类：Q513.2[生物学—生物化学] TN65[电子电信—电路与系统]
作者机构：[1]Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, National Center for TranslationaI Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, Shanghai200240, China, [2]Massachusetts Institute of Technology, Institute for Medical Engineering and Science, Harvard-MIT Division for Health Sciences and Technology, E25-449 Cambridge, Massachusetts, USA, [3]School of Engineering and Materials Science, Queen Mary University of London, E14N5 London, UK, [4]School of Food Science and Environmental Health, College of Science and Health, Dublin Institute of Technology, Camden Row, 4 Dublin, Ireland, [5]Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, Zaragoza 50018, Spain
相关基金：This work was supported by the National Basic Research Program of China （No. 2015CB931802）, National Natural Science Foundation of China （Nos. 81225010, 81327002, 31170961, 20771075, and 20803040）, the National High-tech R＆D Program of China （No. 2014AA020700）, and Special project for nanotechnology from Shanghai （Nos. 13NM1401500 and 15DZ2252000）.

作者： Chenchen Bao[1], Joao Conde[2,3], Fei Pan[1], Chao Li[1], Chunlei Zhang[1], Furong Tian[4], Shujing Liang[1], Jesus M. de la Fuente[1,5], Daxiang Cui[1]

关键词：光声成像, 原位, 体内, 纳米, 热疗, 平台, 棱柱, 单克隆抗体, gold nanoprisms,in situ gastric cancer,photoacoustic imaging,photothermal therapy

中文摘要：

高分辨率的 nanosized photoacoustic 对比代理人的发展是令人激动的还挑战性的工艺的进展。此处，抗体(胸联系癌症的抗原 1 (Brcaa1 ) monoclonal 抗体)- 并且肽(RGD )-functionalized 金牌 nanoprisms (AuNprs ) 被用作组合方法论为在使用的 situ photoacoustic 成像， angiography，和局部性的过高热， orthotopic 和下的鼠科的胃的癌当模特儿。结合 RGD 的 PEGylated AuNprs 为结合抗体的 PEGylated AuNprs 被用于指向的肿瘤 angiography，和 Brcaa1 monoclonal 并且为是可得到的在在 orthotopic 的胃的癌的 situ 成像，肿瘤当模特儿。在 situ photoacoustic 成像允许在肿瘤地点的解剖、功能的成像。在 vivo 肿瘤 angiography，成像以一种时间依赖者方式显示出 photoacoustic 信号的改进。而且， photoacoustic 成像证明肿瘤容器清楚地在局部性的过高热以后被损坏。这作为高度敏感的对比和治疗学的代理人是用二根 AuNprs 探针的第一 proof-of-concept 为在 situ 肿瘤察觉和抑制。这些聪明的抗体 / 肽 AuNprs 能被用作一个有效 nanotheranostic 平台为在局部性的过高热处理以后在有高敏感的 vivo 肿瘤察觉，以及为指向治疗，与单个剂量的注射，它导致肿瘤的肿瘤缩放减小和增加老鼠幸存。

英文摘要：

The development of high-resolution nanosized photoacoustic contrast agents is an exciting yet challenging technological advance. Herein, antibody （breast cancer-associated antigen I （Brcaal） monoclonal antibody）- and peptide （RGD）- functionalized gold nanoprisms （AuNprs） were used as a combinatorial methodology for in situ photoacoustic imaging, angiography, and localized hyperthermia using orthotopic and subcutaneous murine gastric carcinoma models. RGD-conjugated PEGylated AuNprs are available for tumor angiography, and Brcaal monodonal antibody-conjugated PEGylated AuNprs are used for targeting and for in situ imaging of gastric carcinoma in orthotopic tumor models. In situ photoacoustic imaging allowed for anatomical and functional imaging at the tumor site. In vivo tumor angiography imaging showed enhancement of the photoacoustic signal in a time-dependent manner. Furthermore, photoacoustic imaging demonstrated that tumor vessels were clearly damaged after localized hyperthermia. This is the first proof-of-concept using two AuNprs probes as highly sensitive contrasts and therapeutic agents for in situ tumor detection and inhibition. These smart antibody/peptide AuNprs can be used as an efficient nanotheranostic platform for in vivo tumor detection with high sensitivity, as well as for tumor targeting therapy which, with a single-dose injection, results in tumor size reduction and increases mice survival after localized hyperthermia treatment.