中文摘要：

以总黄酮回收率为指标，研究了大孔树脂分离纯化核桃叶总黄酮的工艺。结果表明：LS303型树脂对核桃叶总黄酮有较好的吸附分离性能，是分离纯化核桃叶总黄酮的适宜大孔树脂；分离纯化核桃叶总黄酮的最佳工艺条件为：黄酮量与干树脂量比为1：8，浓度为4．67mg·mL^-1，pH6，4BV的水洗后用4BV的70％乙醇洗脱。经LS303处理后的核桃叶总黄酮回收率在80％以上，纯度70％以上。该工艺简便，能有效分离纯化核桃叶黄酮化合物。

英文摘要：

A method for separation and purification of flavonoids from walnut leaves with macroporous resin was studied, with the recovery rate of flavonoids as index. The results were as flollows： among six types of macroporous resin choosed, resin LS303 was the best one. The optimum process conditions were the loaded amount of flavonoids vs dry resin： 1 ： 8, the extract solution at a concentration about 4.67 mg · mL^-1 , pH 6. After eluted with 4BV of distilled water and 4BV of 70% ethanol, the yield of flavonoids was above 80% and product purity was above 70%. The process is simple, the flavonoids of walnut leaves can be separated and purified effectively.