中文摘要：

目的：比较磷酸化抗原（HDMAPP）、结核杆菌耐热抗原（Mtb-HAg）和结核杆菌低分子耐热抗原Mtb-HAg（10 k）刺激外周血T细胞产生γ-干扰素（IFN-γ）的反应能力方面的特点及差异.方法：采集正常人外周血,分别用HDMAPP、Mtb-HAg和Mtb-HAg（10 k）刺激培养14 h,再加入莫能霉素继续培养6h,收集细胞,加入荧光标记单抗进行表面分子和胞内染色,然后在流式细胞仪上检测各实验组中CD3＋ αβ细胞和CD3＋ γδ细胞胞内IFN-γ的表达.结果：正常人各抗原实验组CD3＋ αβ细胞和CD3＋γδ细胞胞内产生IFN-γ＋细胞比率较阴性组对照组升高（P＜0.01）.各抗原刺激组CD3＋γδ细胞产生IFN-γ＋细胞比率明显高于CD3＋αβ细胞（P ＜0.05 ～P＜0.01）.3种抗原刺激组间CD3＋αβ细胞和CD3＋γδ细胞无明显不同（P＞0.05）.结论：HDMAPP、Mtb-HAg和Mtb-HAg（10 k）均可以刺激外周血中T细胞中αβ和γδ细胞群产生IFN-γ,均可特异性诱导刺激CD3＋γδ细胞产生IFN-γ,HDMAPP、Mtb-HAg（10 k）对CD3＋γδ细胞产生IFN-γ的表达量高于Mtb-HAg.

英文摘要：

Objective： To compare the characteristics and differences of peripheral blood T cells stimulated by HDMAPP, Mycobacterium tuberculosis heat-resistant antigen （Mtb-HAg） and low molecular peptide antigen of Mycobacterium tuberculosis （Mth） （10 k） to produce interferon（IFN-γ）. Methods：The peripheral blood cells from healthy adults were cultured with HDMAPP and Mtb- HAg and Mtb-HAg（ 10 k） for 14 hours, then added Monensin to cultivate for another 6 hours. Cells were collected, and the surface molecule and intracellular cytokines were stained with different fluorochrome-conjugated mAbs. The expressions of IFN-γ, in CD3 ＋ αβ and CD3 ＋ γδ cells of different experiment group were detected by flow cytometry. Results：The ratios of CD3 αβ and CD3 ＋ γδ cells to produce IFN-γ, in all antigen experiment groups were higher than that in control group （ P 〈 0.01 ）. The ratios of CD3 γδ cells to produce IFN-γ in all antigen experiment groups were significantly higher than that of CD3 ＋ αβ cells （ P 〈 0.05 to P 〈 0.01 ）. The differences of CD3 ＋ αβ cells and CD3 ＋γδ cells in three kinds of antigenic stimulation groups were not statistical significance （ P 〉 0.05 ）. Conclusions：CD3 ＋ αβ cells and CD3 ＋ γδ cells can produce IFN-γ by stimulating the peripheral blood T cells with HDMAPP, Mtb-HAg and Mtb-HAg（ 10 k）. CD3 ＋ γδ cells stimulated by three kinds of antigens can produce IFN-7. The IFN-γ level in CD3 ＋γ cells stimulated by HDMAPP and Mtb-HAg（ 10 k） is higher than that of Mtb-HAg stimulating.