中文摘要：

目的：探讨尿酸钠作为佐剂对BALB/c小鼠体液和细胞免疫应答的影响。方法：利用尿酸钠悬浮液为佐剂、天花粉蛋白（TCS）为免疫原对BALB/c小鼠进行免疫，以酶联免疫测定法检测特异性抗体IgG的效价。体外诱导小鼠树突状细胞（DC），流式细胞术分析DC表型，评价尿酸钠体外对DC成熟的效应。以二硝基氟苯建立迟发型超敏反应（DTH）模型，分析尿酸钠在体内对细胞免疫应答的影响。结果：传统弗氏佐剂可极大地增强小鼠对TCS的抗体应答，尿酸钠佐剂对抗体应答不但没有促进，与单独使用免疫原相比，抗体应答反而明显降低。流式细胞术分析显示，尿酸钠对DC表达CD11c和CD83没有影响，但可明显提高MHC II的表达水平。DTH模型中，尿酸钠增强致敏原引发的耳廓肿胀程度，并促进DTH小鼠淋巴细胞的体外增殖能力。结论： 尿酸钠悬浮液作为佐剂，对细胞免疫有显著增强作用，而对体液免疫应答却有一定的抑制作用，提示该佐剂在疫苗研究中有潜在应用前景。

英文摘要：

AIM： To investigate the effects of uric acid sodium salt （UANa） as adjuvant on humoral and cellular immune response in BALB/c mice. METHODS： BALB/c mice were immunized with trichosanthin （TCS） as antigen together with UANa suspension as adjuvant. The antibody titers of IgG were detected by enzyme-linked immunosorbent assay. Dendritic cells （DC） were induced in vitro, the phenotypes of DC were analyzed by flow cytometry and the effect of UANa on DC maturity was evaluated. A delayed-type hypersensitivity （DTH） model was used to analyze the effect of UANa on cellular immune responses in vivo. The in vitro proliferation of lymphocytes was determined by ConA stimulation. RESULTS： Freunds adjuvant greatly enhanced the antibody response of mice to TCS, while UANa adjuvant failed to promote the antibody response but significantly reduced the antibody response as compared to TCS only. No effect of UANa on the expression of CD11c and CD83 in DC was observed by flow cytometry analysis. However, UANa significantly enhanced the expression of MHC II molecule. In the DTH model, UANa enhanced the degree of allergen-induced ear swelling and promoted the ability of lymphocyte proliferation in vitro. CONCLUSION： UANa suspension as adjuvant significantly enhances the cellular immune response but inhibits the humoral immune response to a certain degree, suggesting that UANa has potential application in the vaccine research.