中文摘要：

Two non-coding transcripts of toxins were iso-lated from the venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch, named BmαTX15-SP and BmTXKβ-SP, respectively. Analysis of nucleotide sequences shows that their 5’ sequences are identical to the 5 ’UTR and upstream open reading frame (ORF) sequences of the cDNAs encoding BmαTX15 and BmTXKb, two toxins from Buthus martensii Karsch. But no detectable homology exists in other regions. Two polyadenylated non-coding transcripts encode multiple short ORFs with no more than 34 amino acid resi-dues. The tailing signal (AATAAA) is situated at 14 or 18 bp downstream from the poly(A). The data of genomic se-quences provides support for Bmα TX15-SP and BmTXK β-SP not deriving from splicing errors of pre- mRNAs. Our finding provides evidence for the existence of non-sense-mediated mRNA decay (NMD) pathway in scorpion venom gland cells.

英文摘要：

Two non-coding transcripts of toxins were isolated from the venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch, named BmalphaTX15-SP and BmTXKbeta-SP, respectively. Analysis of nucleotide sequences shows that their 5' sequences are identical to the 5'UTR and upstream open reading frame (ORF) sequences of the cDNAs encoding BmalphaTX15 and BmTXKbeta, two toxins from Buthus martensii Karsch. But no detectable homology exists in other regions. Two polyadenylated non-coding transcripts encode multiple short ORFs with no more than 34 amino acid residues. The tailing signal (AATAAA) is situated at 14 or IS bp downstream from the poly(A). The data of genomic sequences provides support for Bmalpha TX15-SP and BmTXK beta-SP not deriving from splicing errors of pre- mRNAs. Our finding provides evidence for the existence of nonsense-mediated mRNA decay (NMD) pathway in scorpion venom gland cells.