为调查南方红豆杉Taxus chinensis丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)资源的分布情况,从山西、浙江、江西、广东、四川和云南的12个地点采集南方红豆杉根际土样及根样进行AMF孢子的分离、鉴定及侵染率统计。共分离获得AMF 3属36种,其中,无梗囊霉属Acaulospora 13种,占36.1%;球囊霉属Glomus 20种,占55.6%;盾巨孢囊霉属Scutellospora 3种,占8.3%。无梗囊霉属和球囊霉属在12个样地都有分布,而盾巨孢囊霉属仅在浙江-1、浙江-3、浙江-5采样地有分布。空洞无梗囊霉A.cavernata和光壁无梗囊霉A.laevis为南方红豆杉根际的优势种。各采样点孢子密度分布在33–925个/50g干土之间,平均孢子密度为242个/50g干土。各采样点的物种平均丰度10.3种,其中以云南采样点丰度最高,广东采样点丰度最低。各采样点Shannon-Wiener指数和Simpson指数分别在1.12–2.55、0.57–0.91之间。AMF均可侵染不同采样地南方红豆杉根系并形成典型的丛枝-泡囊型菌根,侵染率为18%–82%,侵染级别为2–5级。各采样地天然林中的总球囊霉素和易提取球囊霉素的含量整体高于人工林。
To determine the diversity of arbuscular mycorrhizal fungi (AMF) in the rhizosphere soil of Taxus chinensis, 22 different sites were sa morphologica mpled chara n China. A total of 36 species of AMF belonging to three genera were identified according to the cteristics of the spores isolated from soil samples, in these species, 13 belonged to Acoulosporo, 20 Glomus and 3 Scutellospora, respectively accounting for 36.1%, 55.6% and 8.3% of the total. Acaulospora and Glomus were distributed inall of the 12 sampling sites, but 5cutellospora was only found in Zhejiang-1, were dominant species. The average AMF spore density was 242 per 508 species richness was 10.3. The richness in Yunnan was the highest, and th Zhejiang-3 and Zhejiang-5. A. cavernata and A. laevis air-dried soil, ranging from 33 to 925. The average at in Guangdong the lowest. Shannon-Wiener index and Simpson index ranged respectively from 1.12 to 2.55 and 0.57 to 0.91. Root infection rate ranged from 18%-82% and the nfection level was 2-5. The content of total glomalin (T-GRSP) and easily extractable glomalin (EE-GRSP) in natural forest was higher than that in artificial forest, in general.