利用GPS对吉林省龙井原位保护区野生大豆定点采种,种群L(取样距离设计为50 m)覆盖整个龙井原位保护区。按天然地理分布人为分成A、B、C 3个小种群;同时,在小种群B中取小种群B’(包括32份材料,取样距离设计为18m)。聚类结果表明,90份材料主要划分为两大类群,其它材料亲缘关系较远。龙井原位保护区的野生大豆呈斑块分布,而且小种群间的遗传多样性与地理位置有一定的相关性。25个SSR位点的Simpson指数分布范围是0.3072~0.8393,平均值为0.6788;Shannon-weaver指数分布范围是0.5856~1.9348,平均值为1.3663,表明龙井原位保护区的野生大豆具有较高的遗传多样性。3个小种群的Simpson和Shannon-weaver指数趋势一致,均为A
Wild soybean accessions were collected from Longjing situ conserved region in Jilin province by GPS positioning.Population L,in which 90 wild soybean samples were collected(collection interval was 50 m)covered the whole in-situ conserved region.According to the natural geographical distribution,population L were divided into 3 sub-population A,B and C,respectively;meanwhile,a small sub-population B'(including 32 wild soybean samples,collection interval was 18 m)was taken from sub-population B.Clustering analysis shows that the 90 materials are mainly divided into 2 groups,and the others have a distant relationship,the distribution of the wild soybeans in Longjing conserved region was spotted,a correlation between the genetic diversity and geographical location were found among sub-populations.Simpson index ranged from 0.3072 to 0.8393,with an average of 0.6788.Shannon-weaver index ranged from 0.5856 to 1.9348,with a mean of 1.3663.These results indicated that there existed a highly genetic diversity in Longjing in-situ conserved population of Jilin province,and the genetic diversity of the three sub-populations have the same trend:ABC.The index of subdivision(Fst)among the sub-populations is 0.1419 and the gene flow(Nm)is 1.5112,indicating that 14.19% of genetic variations existed among sub-populations,and a relatively higher frequency of gene flow among the sub-populations.