中文摘要：

目的：探讨芹菜素（ apigenin, Api）在心肌细胞缺氧/复氧损伤中的作用,并阐明Api对心肌损伤的保护作用是否主要由Bcl-2介导。方法培养H9c2心肌样细胞；随机分为正常对照（ Cont ）组、缺氧/复氧（ Anoxia/Reoxygenation, A/R）组、Api预处理组、Api＋ABT-737组。 MTT法检测细胞存活率；Western blot法检测Bcl-2表达；比色法检测培养液LDH活性、细胞SOD及GSH-Px活性、MDA含量；流式细胞仪检测心肌细胞 ROS 含量、线粒体膜电位及细胞凋亡。结果 Api预处理25 h后,心肌细胞Bcl-2表达呈剂量依赖性上调（ P 〈0．01）；细胞存活率升高,培养液 LDH 活性降低,细胞SOD、GSH-Px 活性升高, MDA 含量与 ROS 生成减少,线粒体膜电位更为稳定,细胞凋亡减少（ P〈0．01）；Bcl-2抑制剂ABT-737则可取消Api的上述心肌保护作用。结论Api抗心肌A/R损伤作用涉及Bcl-2信号通路,至少部分依赖于其对Bcl-2表达水平的上调。

英文摘要：

Aim To investigate the relationship be-tween the cardioprotection of apigenin （ Api ） from an-oxia/reoxygenation （ A/R） injury and Bcl-2 pathway. Methods H9 c2 cardiomyocytes were cultured and di-vided into normal control group, A/R group, Api pre-treatment group （ Api ） , Api ＋ Bcl-2 inhibitor group （ Api ＋ ABT-737 ） . Expression of Bcl-2 was deter-mined by Western blot,and cell viability was measured by MTT method. LDH, SOD, GSH-Px, MDA activity were determined by chromometry. ROS generation, mi-tochondrial membrane potential and apoptosis were de-termined by flow cytometry. Results 25h after apige-nin precondition,the expression of Bcl-2 was upregulat-ed in cardiomyocytes （ P 〈0. 01 ） . In the group pre-treated with 40 μmol · L-1 apigenin before A/R, the activity of LDH in culture medium decreased; the ac-tivity of intracellular SOD, GSH-Px increased; the content of MDA and ROS generation decreased; cell viability increased; mitochondrial membrane potential could be more stable and cell apoptosis decreased （ P〈0. 01 ） . However, all these protective effects were attenuated significantly in the group pretreated with apigenin and Bcl-2 inhibitor ABT-737 . Conclusion The effect of apigenin against A/R injury in cardiomyo-cytes involves Bcl-2 pathway, and at least partly de-pends on its effect on upregulating the expression of Bcl-2 .