中文摘要：

从甘肃省天祝县采集白牦牛中寄生的前后盘吸虫经处理后经石蜡切片、HE染色和PAS染色后在显徽镜下观察组织结构。结果表明,前后盘吸虫体壁分为两层,外层为角化的上皮组织,中层为排列密集的环形肌;体腔内主要为生殖系统、消化系统和一些肌束和纤维组织,其中生殖系统包括卵巢、子宫、睾丸,而消化管分布于整个体腔;吸盘明显分为三层,外层、中层和内层,中层细胞较多,呈放射状排列,外层为一层较薄的环形肌,内层为一层较厚的排列整齐的环形肌,还有一层较薄的角化皮肤组织。吸盘的中层所占比例较大,主要是发达的呈放射状排列的肌纤维,靠近内层的地方含有大量的嗜碱性细胞,这些细胞大部分成簇存在,分布在肌纤维之间,也有少量的单个存在,散在分布在肌纤维的中间部位。镜下测量,口吸盘较腹吸盘小,比例大约为1∶1.8。PAS染色结果表明,糖原主要分布在生殖系统中,少量分布于吸盘的肌肉层中。压片结果更有易于观察结构的相对位置。

英文摘要：

To observe the organizational structures of the Paramphistomum from Tianzhu county of Gansu province,paraffin sections were prepared,stained by HE and PAS,and then observed under optical microscopy.In result,the Paramphistomum integumentum were divided into two layers：outer keratinized epithelium and intimal intensive circular muscle.The main part in the body cavity was reproductive system,digestive system and some muscle bundle and fibrous tissues.The reproductive system contained ovaries,uterus and testes.The digestive system spread over the whole body cavity.The sucker was divided into three layers：inner,middle,and outer layers.The middle layer contains more radially arranged cells.The outer layer was relatively thin circular muscle.The inner layer was thicker neatly arranged circular and keratinized skin structure.The middle layer of the sucker accounted for a large proportion,which mainly contained radially arranged muscle fibers.The inner part of middle layer contained a large number of basophilus cell,which distributed between the muscle fibers in cluster.Oral sucker was smaller than ventral sucker,and its proportion was about 1∶1.8.PAS staining revealed that glycogen mainly accumulated in reproductive system,and a few in the muscle layer of acetabulum.The squash technique allows us better observation of relative positions of various structures.