文中通过观察在加有壳聚糖(CS)的超氧自由基中红细胞表面形貌的改变程度采表明CS时超氧自由基的清除效果。由荧光实验中荧光强度的改变来说明不同浓度和不同分子量的CS清除羟基自由基的效率。两种测量手段分别定性地和定量地说明了CS时超氧自由基的清除作用,一致表明CS能有效清除自由基,而且清除效率随着CS浓度的增大而升高。随着CS分子量的增大而降低。
Superoxide radical can destroy the red cell, then cause the change of microstructure. But chitosan can scavenge superoxide radical, so using AFM, the whole surface texture and microstructure of red blood cell can be seen, then the superoxide radical scavenging activity of chitosan is proved through the measurement of the average roughness and the ordering of microstructure; in the fluorescence experiment, the change of fluorescence intensity shows different scavenging activities of chitosan with varied concentration and molecular weight. The two methods draw a conclusion that chitosan can scavenge superoxide radical effectively, and by rising the concentration or reducing the molecular weight, a more effective scavenging activity on superoxide radical can be obtained.