中文摘要：

目的 探讨下咽鳞状细胞癌（hypopharyngeal squamous cell carcinoma,HSCC）中ΔNp63和上皮间质转化（epithelial-mesenchymal transition,EMT）相关分子标志物表达的相关性及临床意义。方法 收集第二军医大学长海医院耳鼻咽喉头颈外科2013年1月至2016年11月收治的98例HSCC患者的肿瘤组织及癌旁正常组织标本。采用免疫组织化学法检测肿瘤组织、癌旁正常组织以及肿瘤不同区域中ΔNp63、E-钙黏蛋白（E-cadherin）、波形蛋白（vimentin）的表达,采用Spearman等级相关检验分析各蛋白表达强度的相关性,采用秩和检验比较各蛋白表达强度与患者临床病理特征的关系。结果 在HSCC组织中ΔNp63、vimentin的表达强度高于癌旁正常上皮组织（P〈0.01）,而E-cadherin的表达强度低于癌旁正常上皮组织（P〈0.01）。与肿瘤中央区相比,肿瘤边缘区可见ΔNp63、E-cadherin的阳性表达强度下降,vimentin的阳性表达强度上升（P均〈0.05）。在HSCC组织中,ΔNp63与E-cadherin的表达强度呈正相关（rs=0.409,P〈0.01）,与vimentin的表达强度呈负相关（rs=-0.333,P〈0.05）。ΔNp63的表达强度与肿瘤T分期、有无淋巴结转移、病理分化程度及临床分期等临床病理特征均无关。E-cadherin和vimentin的表达强度与病理分化程度及有无淋巴结转移有关（P〈0.05）。结论 ΔNp63的异常表达与HSCC的发生、发展有关,其对EMT可能具有抑制作用。

英文摘要：

Objective To investigate the correlation between the expression of △Np63 and epithelial-mesenchymal transition （EMT）-related molecular markers and its clinical significance in hypopharyngeal squamous cell carcinoma （HSCC）. Methods The tumor and adjacent normal epithelium tissues of 98 patients with HSCC were collected from Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Second Military Medical University from Jan. 2013 to Nov. 2016. The expression of △Np63, E-cadherin and vimentin protein in HSCC tissues, adjacent normal epithelium tissues and different regions of HSCC tissues was detected by immunohistochemistry. The correlation between the expression of △Np63, E-cadherin and vimentin proteins was examined by test of Spearman＇s rank-correlation coefficient. The correlation between the expression of proteins and clinicopathological features was evaluated by rank sum test. Results The expression of △Np63 and vimentin in the HSCC tissues was significantly higher than that in the adjacent normal epithelium tissues （P〈0.01）, while the expression of E-cadherin in the HSCC tissues was significantly lower （P〈0.01）. Compared with the central region of HSCC tissues, the positive expression of △Np63 and E-cadherin in the border region was significantly decreased, and the positive expression of vimentin was significantly increased （P〈0.05）. The expression of △Np63 was positively correlated with the expression of E-cadherin （rs=0.409, P〈0.01） and was negatively correlated with the expression of vimentin （rs=-0.333, P〈0.05） in HSCC tissues. There was no significant correlation between △Np63 and T stage, lymph node metastasis, degree of pathological differentiation or other clinicopathological features. The expression of E-cadherin and vimentin was correlated with the degree of pathological differentiation and lymph node metastasis （P〈0.05）. Conclusion Abnormal expression of △Np63 is associated with the occurrence and development of HSCC