中文摘要：

目的 探讨创伤后应激障碍（PTSD）模型大鼠内侧前额皮质（mPFC）磷酸化细胞外信号调节激酶1/2（pERK1/2）和c-fos的表达变化。 方法 将30只成年健康雄性Wistar大鼠随机分为对照组（15只）和PTSD模型组（15只），采用无连续单一应激（SPS）方法制备PTSD模型。用免疫组织化学和免疫印迹法检测mPFC pERK1/2的表达变化，RT-PCR检测c-fos mRNA表达变化。 结果 免疫组织化学分析显示，对照组和模型组pERK1/2阳性细胞数分别为10.4±2.07、48.8±10.08，阳性信号吸光度值分别为24.955±3.691、110.810±10.643，差异有统计学意义（P〈0.01）；免疫印迹分析显示，对照组和模型组pERK/2相对表达量分别为0.510±0.052和1.109±0.106，差异有统计学意义（P〈0.01）；RT-PCR分析结果显示，对照组和模型组c-fos mRNA相对表达分别为0.267±0.067和1.049±0.131，差异有统计学意义（P〈0.01）。 结论 mPFC pERK1/2和c-fos表达增高，可能参与了PTSD模型大鼠的病理生理过程。

英文摘要：

Objective To observe the changes of phosphorylated extracellular signalregulated kinase 1/2 （pERK1/2） and c-fos in the medial prefrontal cortex （mPFC） of post-traumatic stress disorder （PTSD） rats. Methods Male Wistar rats were randomly divided into control group and PTSD model group. The model group rats were exposed to the singleprolonged stress （SPS） to set up the rat PTSD model. The expression of pERK1/2 was detected using immunohistochemistry and Western blotting, and the expression of c-fos mRNA was detected using reverse transcription polymerase chain reaction （RT-PCR）. Results The result of immunohistochemistry analysis showed that the number of pERK1/2-positive cells of control group and model group were 10.4±2.07 and 48.8±10.08 respectively （P〈0.01）, and integral optical density were 24.955±3.691 and 110.810±10.643 respectively （P〈0.01）. And Western blotting showed that relative expression quantities of pERK/β actin were 0.510±0.052 and 1.109±0.106 respectively （P〈0.01）. The quantities of c-fos mRNA relative expression of control group and model group were 0.267±0.067 and 1.049±0.131 （P〈0.01）. Conlusion The levels of pERK1/2 and c-fos increase significantly in mPFC of PTSD model rats. The ERK signal transduction pathway in mPFC might play an important role in the pathogenesis of PTSD.