中文摘要：

目的：探讨Nanog在FSH诱导的卵巢癌转移和侵袭中的作用。方法：采用Western blot法检测永生化卵巢上皮细胞Moody,良性卵巢肿瘤细胞Mcv152和卵巢癌细胞Hey、Ho8910、SKOV3中Nanog表达。使用FSH以浓度梯度和时间梯度的方式处理SKOV3细胞,Western blot法检测Nanog蛋白表达。使用FSH处理si-Con或si-Nanog转染卵巢癌SKOV3细胞,实验分组：0.1%DMSO＋转染空白干扰RNA组（Con＋si-Con）、FSH＋转染空白干扰RNA组（FSH＋si-Con）、0.1%DMSO＋转染Nanog干扰RNA组（Con＋siNanog）和FSH＋转染Nanog干扰RNA组（FSH＋si-Nanog）。使用划痕实验和Transwell侵袭实验检测4组细胞的转移和侵袭能力,Western blot法分析4组细胞中Nanog蛋白表达。应用免疫组化法检测卵巢癌组织中Nanog蛋白表达情况。结果：卵巢癌细胞系Hey、Ho8910、SKOV3中Nanog表达明显高于永生化和良性卵巢上皮细胞系（P〈0.05）。FSH上调Nanog表达以浓度和时间依赖的方式。对照组（Con＋si-Con,Con＋si-Nanog）的迁移和侵袭能力明显低于实验组（FSH＋si-Con,FSH＋si-Nanog）（P〈0.05）。卵巢癌组织中Nanog表达水平明显高于正常卵巢组织（P〈0.05）,Nanog蛋白表达与卵巢癌转移相关。结论：Nanog是FSH重要的下游靶基因,介导FSH诱导的卵巢癌转移作用。

英文摘要：

Objective：To investigate the role of Nanog in FSH-induced ovarian cancer metastasis by siRNA interference Nanog expression. Methods： The expression of Nanog inimmortalized ovarian epithelial cell line Moody,benign ovarian tumor cell line Mcv-152 and ovarian cancer cell lines Hey,Ho8910,SKOV3 were analyzed through Western blot. The protein expression of Nanog in SKOV3 treated with FSH in dose-and time-dependent manner was detected by Western blot. FSH treated SKOV3 cells transfected with si-Con or si-Nanog. Four groups were designed： control group（ Con ＋ si-Con,Con ＋ si-Nanog）,experimental group（ FSH ＋ siCon,FSH ＋ si-Nanog）. The ability of migration and invasion and the expression of Nanog in four groups of cells were examined through wound healing assay,transwell assay and Western blot. Immunohistochemistry was used to detect Nanog protein expression in ovarian cancer tissues. Results：Nanog expressions in ovarian cancer cell lines Hey,Ho8910,SKOV3 were significantly higher than that in immortalized and benign ovarian epithelial cell lines（ P〈0.05）. FSH upregulated Nanog expression in dose-and time-dependent manner. The ability of migration and invasionin control group（ Con ＋ si-Con,Con ＋ si-Nanog） was significantly lower than that in ex-perimental group（ FSH ＋ si-Con,FSH ＋ si-Nanog）（ P〈0. 05）. Nanog expression in ovarian cancer tissues was significantly higher than normal ovarian tissues（ P〈0. 05）,which was positively correlated with metastasis in ovarian cnacer. Conclusions：Nanog is an important downstream target gene for FSH,which mediates FSH-induced migration and invasion in ovarian cnacer.